Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28115 | 5' | -51.6 | NC_005887.1 | + | 38824 | 0.66 | 0.851511 |
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Target: 5'- gUCGuucCGGGAAGG-CGAUuUCAUCa- -3' miRNA: 3'- -GGCuu-GCCCUUCCaGCUGuAGUAGca -5' |
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| 28115 | 5' | -51.6 | NC_005887.1 | + | 10983 | 0.67 | 0.842689 |
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Target: 5'- gUGAGCaGGccGGcCGACGUCAUCu- -3' miRNA: 3'- gGCUUGcCCuuCCaGCUGUAGUAGca -5' |
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| 28115 | 5' | -51.6 | NC_005887.1 | + | 24002 | 0.68 | 0.795278 |
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Target: 5'- gCGcaGACGGccgcGAGGcCGACGUCAUCGc -3' miRNA: 3'- gGC--UUGCCc---UUCCaGCUGUAGUAGCa -5' |
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| 28115 | 5' | -51.6 | NC_005887.1 | + | 23242 | 0.68 | 0.785212 |
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Target: 5'- gCGAGCGGcGgcGGUCGGCGcgCAaaaggcgcagcUCGUg -3' miRNA: 3'- gGCUUGCC-CuuCCAGCUGUa-GU-----------AGCA- -5' |
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| 28115 | 5' | -51.6 | NC_005887.1 | + | 3880 | 0.68 | 0.785212 |
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Target: 5'- gCGAACGGGAAGGUgCGAaagCcgCu- -3' miRNA: 3'- gGCUUGCCCUUCCA-GCUguaGuaGca -5' |
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| 28115 | 5' | -51.6 | NC_005887.1 | + | 16490 | 0.68 | 0.754054 |
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Target: 5'- uCCG-GCGGGcuGGUCGGCGaUGUCGc -3' miRNA: 3'- -GGCuUGCCCuuCCAGCUGUaGUAGCa -5' |
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| 28115 | 5' | -51.6 | NC_005887.1 | + | 35044 | 0.68 | 0.742316 |
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Target: 5'- -gGAAUGGGGuuacgcgAGGUCGcugcCGUCGUCGg -3' miRNA: 3'- ggCUUGCCCU-------UCCAGCu---GUAGUAGCa -5' |
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| 28115 | 5' | -51.6 | NC_005887.1 | + | 4280 | 0.7 | 0.677343 |
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Target: 5'- cCCGAugaucGCGcGcGAGGUCGACAUCcUCGc -3' miRNA: 3'- -GGCU-----UGC-CcUUCCAGCUGUAGuAGCa -5' |
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| 28115 | 5' | -51.6 | NC_005887.1 | + | 33999 | 0.71 | 0.620937 |
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Target: 5'- gCCGAGCGccgucGGcAGGUCGACGUCGa--- -3' miRNA: 3'- -GGCUUGC-----CCuUCCAGCUGUAGUagca -5' |
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| 28115 | 5' | -51.6 | NC_005887.1 | + | 6186 | 1.09 | 0.002076 |
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Target: 5'- cCCGAACGGGAAGGUCGACAUCAUCGUc -3' miRNA: 3'- -GGCUUGCCCUUCCAGCUGUAGUAGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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