Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28123 | 5' | -58.4 | NC_005887.1 | + | 9103 | 1.1 | 0.000343 |
Target: 5'- cGGGCACCGGCGCCGUUACUCUGGUCGc -3' miRNA: 3'- -CCCGUGGCCGCGGCAAUGAGACCAGC- -5' |
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28123 | 5' | -58.4 | NC_005887.1 | + | 33528 | 0.66 | 0.517484 |
Target: 5'- cGGCgaucACCGugaGCGCC--UGCuUCUGGUCGg -3' miRNA: 3'- cCCG----UGGC---CGCGGcaAUG-AGACCAGC- -5' |
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28123 | 5' | -58.4 | NC_005887.1 | + | 38305 | 0.66 | 0.516444 |
Target: 5'- aGGGUGCCGuCGCCGUUgucgaucaccgGCUCaggaaggUGGUgGg -3' miRNA: 3'- -CCCGUGGCcGCGGCAA-----------UGAG-------ACCAgC- -5' |
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28123 | 5' | -58.4 | NC_005887.1 | + | 40766 | 0.66 | 0.466629 |
Target: 5'- uGGcGCGCCGcGCGCCGccgaGC-CggcGGUCGa -3' miRNA: 3'- -CC-CGUGGC-CGCGGCaa--UGaGa--CCAGC- -5' |
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28123 | 5' | -58.4 | NC_005887.1 | + | 33983 | 0.66 | 0.466629 |
Target: 5'- -aGCACCGGCGCCGUaucacGCUCa----- -3' miRNA: 3'- ccCGUGGCCGCGGCAa----UGAGaccagc -5' |
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28123 | 5' | -58.4 | NC_005887.1 | + | 21266 | 0.67 | 0.456767 |
Target: 5'- gGGGCACCgcggcGGCGgCGUaUGCgcugCUGG-CGu -3' miRNA: 3'- -CCCGUGG-----CCGCgGCA-AUGa---GACCaGC- -5' |
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28123 | 5' | -58.4 | NC_005887.1 | + | 18551 | 0.69 | 0.331876 |
Target: 5'- uGGGCGgCGGCGCCGgc-UUCgcgGG-CGa -3' miRNA: 3'- -CCCGUgGCCGCGGCaauGAGa--CCaGC- -5' |
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28123 | 5' | -58.4 | NC_005887.1 | + | 41727 | 0.8 | 0.060626 |
Target: 5'- cGGGCGgcgauccgaucCCGGCGCgCGg-GCUCUGGUCGa -3' miRNA: 3'- -CCCGU-----------GGCCGCG-GCaaUGAGACCAGC- -5' |
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28123 | 5' | -58.4 | NC_005887.1 | + | 21579 | 0.68 | 0.373479 |
Target: 5'- cGGCAgCCGGCGUCGUUGCgccgauugCcGG-CGg -3' miRNA: 3'- cCCGU-GGCCGCGGCAAUGa-------GaCCaGC- -5' |
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28123 | 5' | -58.4 | NC_005887.1 | + | 792 | 0.68 | 0.382214 |
Target: 5'- -aGCACCGGUGCUGgUACgagaucagCUGGUUc -3' miRNA: 3'- ccCGUGGCCGCGGCaAUGa-------GACCAGc -5' |
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28123 | 5' | -58.4 | NC_005887.1 | + | 12479 | 0.67 | 0.40922 |
Target: 5'- -cGCGCgCGGUGUCGUUGCccugCUGGUa- -3' miRNA: 3'- ccCGUG-GCCGCGGCAAUGa---GACCAgc -5' |
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28123 | 5' | -58.4 | NC_005887.1 | + | 7339 | 0.67 | 0.418482 |
Target: 5'- cGGGCgGCCGGgGCCGaUG-UCgcGGUCGc -3' miRNA: 3'- -CCCG-UGGCCgCGGCaAUgAGa-CCAGC- -5' |
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28123 | 5' | -58.4 | NC_005887.1 | + | 9159 | 0.67 | 0.427872 |
Target: 5'- cGGCGCCGGUGCCc--GCgCUGG-Ca -3' miRNA: 3'- cCCGUGGCCGCGGcaaUGaGACCaGc -5' |
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28123 | 5' | -58.4 | NC_005887.1 | + | 4682 | 0.66 | 0.465638 |
Target: 5'- cGGcCACCGGCGCCGcggcgagcaucugUUGCaUCUGcG-CGg -3' miRNA: 3'- cCC-GUGGCCGCGGC-------------AAUG-AGAC-CaGC- -5' |
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28123 | 5' | -58.4 | NC_005887.1 | + | 22145 | 0.66 | 0.476601 |
Target: 5'- aGGGCcccgaCGGcCGCCGUg---CUGGUUGu -3' miRNA: 3'- -CCCGug---GCC-GCGGCAaugaGACCAGC- -5' |
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28123 | 5' | -58.4 | NC_005887.1 | + | 36103 | 0.71 | 0.252677 |
Target: 5'- aGGCcuugcgccucGCCGGCGCCGaggugACgcugCUGGUUGa -3' miRNA: 3'- cCCG----------UGGCCGCGGCaa---UGa---GACCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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