Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28124 | 3' | -60.3 | NC_005887.1 | + | 26204 | 0.71 | 0.18156 |
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Target: 5'- aACGG-GGCGGCGGugG--CCGGCCu -3' miRNA: 3'- aUGCCaCCGCUGCUugCugGGCCGGc -5' |
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| 28124 | 3' | -60.3 | NC_005887.1 | + | 34389 | 0.71 | 0.176817 |
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Target: 5'- gUGCGGccgGGCG-CGA--GGCCCGGCCu -3' miRNA: 3'- -AUGCCa--CCGCuGCUugCUGGGCCGGc -5' |
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| 28124 | 3' | -60.3 | NC_005887.1 | + | 7366 | 0.72 | 0.167662 |
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Target: 5'- gACGGacGGCGACacGACGACCCacagcgggcGGCCGg -3' miRNA: 3'- aUGCCa-CCGCUGc-UUGCUGGG---------CCGGC- -5' |
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| 28124 | 3' | -60.3 | NC_005887.1 | + | 13212 | 0.72 | 0.163246 |
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Target: 5'- cGCGGcaaGCGACGAGacgaGGCCCGGCaCGc -3' miRNA: 3'- aUGCCac-CGCUGCUUg---CUGGGCCG-GC- -5' |
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| 28124 | 3' | -60.3 | NC_005887.1 | + | 13379 | 0.74 | 0.111589 |
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Target: 5'- aUGCGGccGGCGuugcCGAACG-CCUGGCCGa -3' miRNA: 3'- -AUGCCa-CCGCu---GCUUGCuGGGCCGGC- -5' |
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| 28124 | 3' | -60.3 | NC_005887.1 | + | 9475 | 1.07 | 0.000371 |
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Target: 5'- gUACGGUGGCGACGAACGACCCGGCCGa -3' miRNA: 3'- -AUGCCACCGCUGCUUGCUGGGCCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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