Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28129 | 3' | -58.6 | NC_005887.1 | + | 21375 | 0.66 | 0.476601 |
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Target: 5'- cGGCGCggugggcGugCC-CGUGAAUGuGGGc -3' miRNA: 3'- uCCGCGa------CugGGaGCACUUGCuCCCc -5' |
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| 28129 | 3' | -58.6 | NC_005887.1 | + | 3948 | 0.66 | 0.472599 |
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Target: 5'- cGGCGCgcagaagcucgucGACCCgccguugcUCGcGAACGAGGaGGc -3' miRNA: 3'- uCCGCGa------------CUGGG--------AGCaCUUGCUCC-CC- -5' |
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| 28129 | 3' | -58.6 | NC_005887.1 | + | 41855 | 0.66 | 0.466629 |
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Target: 5'- cGGCGcCUGAUgCUCGUGccguuCGAGcGGa -3' miRNA: 3'- uCCGC-GACUGgGAGCACuu---GCUC-CCc -5' |
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| 28129 | 3' | -58.6 | NC_005887.1 | + | 17692 | 0.68 | 0.34563 |
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Target: 5'- cGGCGCUGACCgUCGUGcgcagcGuuuccaucuugcgcGCGAGcgcGGGa -3' miRNA: 3'- uCCGCGACUGGgAGCAC------U--------------UGCUC---CCC- -5' |
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| 28129 | 3' | -58.6 | NC_005887.1 | + | 12876 | 0.7 | 0.265828 |
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Target: 5'- cGGGCucaGCUGcuucCCCUCGUucACGAGGGu -3' miRNA: 3'- -UCCG---CGACu---GGGAGCAcuUGCUCCCc -5' |
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| 28129 | 3' | -58.6 | NC_005887.1 | + | 12794 | 1.1 | 0.000304 |
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Target: 5'- cAGGCGCUGACCCUCGUGAACGAGGGGa -3' miRNA: 3'- -UCCGCGACUGGGAGCACUUGCUCCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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