Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28132 | 3' | -55.1 | NC_005887.1 | + | 10188 | 0.69 | 0.426758 |
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Target: 5'- --uCUUCGUCGUacgugcagccgaucaGCUGGCcgucGGCGCGGg -3' miRNA: 3'- cauGAAGUAGCGa--------------CGACCG----UCGCGCC- -5' |
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| 28132 | 3' | -55.1 | NC_005887.1 | + | 3395 | 0.7 | 0.406458 |
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Target: 5'- -cGCUguucgCcgCGCgcuaccGCUGGCAGgGCGGc -3' miRNA: 3'- caUGAa----GuaGCGa-----CGACCGUCgCGCC- -5' |
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| 28132 | 3' | -55.1 | NC_005887.1 | + | 9821 | 0.7 | 0.406458 |
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Target: 5'- -cGCgaacgCGUCGCaGCcGGuCAGCGCGGc -3' miRNA: 3'- caUGaa---GUAGCGaCGaCC-GUCGCGCC- -5' |
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| 28132 | 3' | -55.1 | NC_005887.1 | + | 14104 | 0.7 | 0.397007 |
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Target: 5'- -gGCaUCGUCucagGCUGCgccGGCGGCGCGc -3' miRNA: 3'- caUGaAGUAG----CGACGa--CCGUCGCGCc -5' |
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| 28132 | 3' | -55.1 | NC_005887.1 | + | 11207 | 0.7 | 0.397007 |
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Target: 5'- -gGCUUCGUCGCcGaaGGCGuGCGcCGGa -3' miRNA: 3'- caUGAAGUAGCGaCgaCCGU-CGC-GCC- -5' |
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| 28132 | 3' | -55.1 | NC_005887.1 | + | 23474 | 0.7 | 0.378535 |
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Target: 5'- -aGCUgaUCGacgCGCUGCUgcggcugccagcGGCAGCGCGa -3' miRNA: 3'- caUGA--AGUa--GCGACGA------------CCGUCGCGCc -5' |
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| 28132 | 3' | -55.1 | NC_005887.1 | + | 14697 | 0.7 | 0.369518 |
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Target: 5'- -gGC-UCGUCGCggcggGCgGGCcgGGCGCGGc -3' miRNA: 3'- caUGaAGUAGCGa----CGaCCG--UCGCGCC- -5' |
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| 28132 | 3' | -55.1 | NC_005887.1 | + | 18609 | 0.73 | 0.259108 |
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Target: 5'- uUACggu-UCGUUGCUcGGCAGCGCGa -3' miRNA: 3'- cAUGaaguAGCGACGA-CCGUCGCGCc -5' |
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| 28132 | 3' | -55.1 | NC_005887.1 | + | 11784 | 0.76 | 0.177153 |
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Target: 5'- -gGCgUCcUCGCUGCUcGGCGGCGuCGGu -3' miRNA: 3'- caUGaAGuAGCGACGA-CCGUCGC-GCC- -5' |
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| 28132 | 3' | -55.1 | NC_005887.1 | + | 13601 | 1.1 | 0.000583 |
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Target: 5'- cGUACUUCAUCGCUGCUGGCAGCGCGGc -3' miRNA: 3'- -CAUGAAGUAGCGACGACCGUCGCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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