Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28134 | 3' | -50.4 | NC_005887.1 | + | 30685 | 0.71 | 0.698397 |
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Target: 5'- uCCGGAUCUG-------CCGGACGCGCu -3' miRNA: 3'- -GGUCUAGAUacagcuaGGCCUGUGCG- -5' |
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| 28134 | 3' | -50.4 | NC_005887.1 | + | 22900 | 0.7 | 0.710679 |
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Target: 5'- aCCgAGAUCUGccgGUUGAgcacgUUCGGAUGCGCc -3' miRNA: 3'- -GG-UCUAGAUa--CAGCU-----AGGCCUGUGCG- -5' |
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| 28134 | 3' | -50.4 | NC_005887.1 | + | 19888 | 0.67 | 0.885452 |
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Target: 5'- gCCAG-UgUGUcGUCGAcgcccccguuUCCGGuCGCGCc -3' miRNA: 3'- -GGUCuAgAUA-CAGCU----------AGGCCuGUGCG- -5' |
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| 28134 | 3' | -50.4 | NC_005887.1 | + | 13190 | 0.67 | 0.885452 |
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Target: 5'- cCCGGcacgcUCgagGUGUCGAccUUCGG-CACGCc -3' miRNA: 3'- -GGUCu----AGa--UACAGCU--AGGCCuGUGCG- -5' |
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| 28134 | 3' | -50.4 | NC_005887.1 | + | 28999 | 0.67 | 0.893006 |
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Target: 5'- gCCAGugcucgCUGUgcGUCGAcgcaaUGGGCGCGCg -3' miRNA: 3'- -GGUCua----GAUA--CAGCUag---GCCUGUGCG- -5' |
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| 28134 | 3' | -50.4 | NC_005887.1 | + | 10685 | 0.66 | 0.907257 |
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Target: 5'- cCCAGG-CUccgcgagaagcGUGUCGcgauGUUCGuGACGCGCu -3' miRNA: 3'- -GGUCUaGA-----------UACAGC----UAGGC-CUGUGCG- -5' |
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| 28134 | 3' | -50.4 | NC_005887.1 | + | 7655 | 0.66 | 0.920341 |
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Target: 5'- aCCGGcgCcgcuuacgcgAUGcCG-UCCGGAUACGCc -3' miRNA: 3'- -GGUCuaGa---------UACaGCuAGGCCUGUGCG- -5' |
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| 28134 | 3' | -50.4 | NC_005887.1 | + | 41236 | 0.66 | 0.924641 |
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Target: 5'- gCGGAUC-AUGuUCGAcaccauugcggcuuUCUGG-CACGCg -3' miRNA: 3'- gGUCUAGaUAC-AGCU--------------AGGCCuGUGCG- -5' |
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| 28134 | 3' | -50.4 | NC_005887.1 | + | 26786 | 0.75 | 0.425339 |
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Target: 5'- gCCcGGUCUAUGUCGAgcugcCCGcGGcCGCGCg -3' miRNA: 3'- -GGuCUAGAUACAGCUa----GGC-CU-GUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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