Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28134 | 3' | -50.4 | NC_005887.1 | + | 42122 | 0.7 | 0.732742 |
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Target: 5'- -aGGAcUUGUGgCGAUCCuGGCGCGCa -3' miRNA: 3'- ggUCUaGAUACaGCUAGGcCUGUGCG- -5' |
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| 28134 | 3' | -50.4 | NC_005887.1 | + | 22900 | 0.7 | 0.710679 |
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Target: 5'- aCCgAGAUCUGccgGUUGAgcacgUUCGGAUGCGCc -3' miRNA: 3'- -GG-UCUAGAUa--CAGCU-----AGGCCUGUGCG- -5' |
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| 28134 | 3' | -50.4 | NC_005887.1 | + | 30685 | 0.71 | 0.698397 |
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Target: 5'- uCCGGAUCUG-------CCGGACGCGCu -3' miRNA: 3'- -GGUCUAGAUacagcuaGGCCUGUGCG- -5' |
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| 28134 | 3' | -50.4 | NC_005887.1 | + | 2330 | 0.73 | 0.586003 |
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Target: 5'- gCUGGcAUCUAUGgCGAUCCGG-CGgGCg -3' miRNA: 3'- -GGUC-UAGAUACaGCUAGGCCuGUgCG- -5' |
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| 28134 | 3' | -50.4 | NC_005887.1 | + | 24920 | 0.73 | 0.574709 |
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Target: 5'- gCCGGAUCggcaccguUGUCGAUC-GcGACGCGUg -3' miRNA: 3'- -GGUCUAGau------ACAGCUAGgC-CUGUGCG- -5' |
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| 28134 | 3' | -50.4 | NC_005887.1 | + | 22623 | 0.74 | 0.508297 |
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Target: 5'- aUCAGcGUCUcgggcAUGUCGAUCgCGGACggcauGCGCa -3' miRNA: 3'- -GGUC-UAGA-----UACAGCUAG-GCCUG-----UGCG- -5' |
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| 28134 | 3' | -50.4 | NC_005887.1 | + | 14509 | 0.75 | 0.476304 |
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Target: 5'- gCAcGGUCUG-GUCGAUCgCGGuuGCGCGCg -3' miRNA: 3'- gGU-CUAGAUaCAGCUAG-GCC--UGUGCG- -5' |
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| 28134 | 3' | -50.4 | NC_005887.1 | + | 26786 | 0.75 | 0.425339 |
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Target: 5'- gCCcGGUCUAUGUCGAgcugcCCGcGGcCGCGCg -3' miRNA: 3'- -GGuCUAGAUACAGCUa----GGC-CU-GUGCG- -5' |
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| 28134 | 3' | -50.4 | NC_005887.1 | + | 14271 | 1.15 | 0.001234 |
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Target: 5'- gCCAGAUCUAUGUCGAUCCGGACACGCu -3' miRNA: 3'- -GGUCUAGAUACAGCUAGGCCUGUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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