Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 28138 | 5' | -55.9 | NC_005887.1 | + | 32293 | 0.71 | 0.314616 |
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Target: 5'- gGGCGAUCGucGGGCcAGCCgGCGCGc-- -3' miRNA: 3'- -UCGCUAGC--UCCGcUUGGaCGCGCuuc -5' |
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| 28138 | 5' | -55.9 | NC_005887.1 | + | 15849 | 0.72 | 0.277162 |
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Target: 5'- cGCGAUCGAGG-GAaacGCCcGCGCGcAGc -3' miRNA: 3'- uCGCUAGCUCCgCU---UGGaCGCGCuUC- -5' |
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| 28138 | 5' | -55.9 | NC_005887.1 | + | 30683 | 0.72 | 0.299191 |
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Target: 5'- gGGCaGAUCGAccucguacucGGCGAGCaggUGCGCGAGc -3' miRNA: 3'- -UCG-CUAGCU----------CCGCUUGg--ACGCGCUUc -5' |
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| 28138 | 5' | -55.9 | NC_005887.1 | + | 40746 | 0.73 | 0.243353 |
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Target: 5'- cGCG-UCGAGGCGAucGCCguugGCGCGc-- -3' miRNA: 3'- uCGCuAGCUCCGCU--UGGa---CGCGCuuc -5' |
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| 28138 | 5' | -55.9 | NC_005887.1 | + | 34544 | 0.76 | 0.157214 |
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Target: 5'- gGGCGAcagcuucUCGGGcGCGAGCC-GCGCGAAc -3' miRNA: 3'- -UCGCU-------AGCUC-CGCUUGGaCGCGCUUc -5' |
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| 28138 | 5' | -55.9 | NC_005887.1 | + | 16174 | 1.09 | 0.000605 |
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Target: 5'- gAGCGAUCGAGGCGAACCUGCGCGAAGa -3' miRNA: 3'- -UCGCUAGCUCCGCUUGGACGCGCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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