Results 41 - 46 of 46 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio![]() |
P value |
Predicted miRNA align pattern | |||||||
28138 | 5' | -55.9 | NC_005887.1 | + | 34929 | 0.66 | 0.620242 |
Target: 5'- aGGCGAUCGAGGCcgcGAUCaagucgguUGcCGuCGAAGg -3' miRNA: 3'- -UCGCUAGCUCCGc--UUGG--------AC-GC-GCUUC- -5' |
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28138 | 5' | -55.9 | NC_005887.1 | + | 2501 | 0.66 | 0.609086 |
Target: 5'- uAGCGcgCGAGGUGucauUCgucgGCGCGGGc -3' miRNA: 3'- -UCGCuaGCUCCGCuu--GGa---CGCGCUUc -5' |
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28138 | 5' | -55.9 | NC_005887.1 | + | 32450 | 0.66 | 0.59795 |
Target: 5'- gAGU-AUCGAGGaUGucaaccugauGACCUGCGCGGAu -3' miRNA: 3'- -UCGcUAGCUCC-GC----------UUGGACGCGCUUc -5' |
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28138 | 5' | -55.9 | NC_005887.1 | + | 35480 | 0.66 | 0.575778 |
Target: 5'- -cUGAUCGAcucgugGGCGAagGCCgUGCGCGGGc -3' miRNA: 3'- ucGCUAGCU------CCGCU--UGG-ACGCGCUUc -5' |
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28138 | 5' | -55.9 | NC_005887.1 | + | 37805 | 0.66 | 0.59795 |
Target: 5'- gGGCGGgcUCGuGGaacuucuCCUGCGCGAAu -3' miRNA: 3'- -UCGCU--AGCuCCgcuu---GGACGCGCUUc -5' |
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28138 | 5' | -55.9 | NC_005887.1 | + | 18034 | 0.66 | 0.575778 |
Target: 5'- uGCGGcCGAugccGGCGAGCCcGCGCa--- -3' miRNA: 3'- uCGCUaGCU----CCGCUUGGaCGCGcuuc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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