Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28138 | 5' | -55.9 | NC_005887.1 | + | 37805 | 0.66 | 0.59795 |
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Target: 5'- gGGCGGgcUCGuGGaacuucuCCUGCGCGAAu -3' miRNA: 3'- -UCGCU--AGCuCCgcuu---GGACGCGCUUc -5' |
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| 28138 | 5' | -55.9 | NC_005887.1 | + | 29772 | 0.66 | 0.586845 |
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Target: 5'- uGCGAcgCGAcGGCGAguGgCUGCGCGu-- -3' miRNA: 3'- uCGCUa-GCU-CCGCU--UgGACGCGCuuc -5' |
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| 28138 | 5' | -55.9 | NC_005887.1 | + | 18034 | 0.66 | 0.575778 |
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Target: 5'- uGCGGcCGAugccGGCGAGCCcGCGCa--- -3' miRNA: 3'- uCGCUaGCU----CCGCUUGGaCGCGcuuc -5' |
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| 28138 | 5' | -55.9 | NC_005887.1 | + | 35480 | 0.66 | 0.575778 |
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Target: 5'- -cUGAUCGAcucgugGGCGAagGCCgUGCGCGGGc -3' miRNA: 3'- ucGCUAGCU------CCGCU--UGG-ACGCGCUUc -5' |
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| 28138 | 5' | -55.9 | NC_005887.1 | + | 16064 | 0.67 | 0.532071 |
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Target: 5'- cGGC--UCGcGGCGggUCUGCGCGc-- -3' miRNA: 3'- -UCGcuAGCuCCGCuuGGACGCGCuuc -5' |
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| 28138 | 5' | -55.9 | NC_005887.1 | + | 34544 | 0.76 | 0.157214 |
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Target: 5'- gGGCGAcagcuucUCGGGcGCGAGCC-GCGCGAAc -3' miRNA: 3'- -UCGCU-------AGCUC-CGCUUGGaCGCGCUUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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