Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28138 | 5' | -55.9 | NC_005887.1 | + | 8458 | 0.67 | 0.553797 |
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Target: 5'- cGCGAUCGAgcgcccGGCGcAACCgcucgGCGaCGAc- -3' miRNA: 3'- uCGCUAGCU------CCGC-UUGGa----CGC-GCUuc -5' |
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| 28138 | 5' | -55.9 | NC_005887.1 | + | 2413 | 0.67 | 0.553797 |
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Target: 5'- uGGCu-UCGAGGCGAaGCCcGCGcCGAc- -3' miRNA: 3'- -UCGcuAGCUCCGCU-UGGaCGC-GCUuc -5' |
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| 28138 | 5' | -55.9 | NC_005887.1 | + | 1197 | 0.67 | 0.542898 |
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Target: 5'- uGGCGAucuaaacaaaacUCGGaucaccuuaucuGGCGGGCgCUGCGCGGc- -3' miRNA: 3'- -UCGCU------------AGCU------------CCGCUUG-GACGCGCUuc -5' |
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| 28138 | 5' | -55.9 | NC_005887.1 | + | 41674 | 0.67 | 0.532071 |
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Target: 5'- uGGCGAgccggaCGAagguaGCGAG-CUGCGCGAGGg -3' miRNA: 3'- -UCGCUa-----GCUc----CGCUUgGACGCGCUUC- -5' |
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| 28138 | 5' | -55.9 | NC_005887.1 | + | 4888 | 0.67 | 0.532071 |
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Target: 5'- cGCGA-CGAuGGUcgccgaGGGCCgGCGCGAGGu -3' miRNA: 3'- uCGCUaGCU-CCG------CUUGGaCGCGCUUC- -5' |
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| 28138 | 5' | -55.9 | NC_005887.1 | + | 16174 | 1.09 | 0.000605 |
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Target: 5'- gAGCGAUCGAGGCGAACCUGCGCGAAGa -3' miRNA: 3'- -UCGCUAGCUCCGCUUGGACGCGCUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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