Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2814 | 5' | -57 | NC_001491.2 | + | 142996 | 0.68 | 0.752359 |
Target: 5'- gGGgCGuGGCUUGAgacaGCUGUGuGGGGGGa -3' miRNA: 3'- -CCgGC-UCGGACUa---CGACGCuUCCUCC- -5' |
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2814 | 5' | -57 | NC_001491.2 | + | 144673 | 0.7 | 0.663513 |
Target: 5'- cGGCCGcGCUg---GCUGCGcggauGAGGGGGu -3' miRNA: 3'- -CCGGCuCGGacuaCGACGC-----UUCCUCC- -5' |
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2814 | 5' | -57 | NC_001491.2 | + | 147511 | 0.67 | 0.852059 |
Target: 5'- gGGCCcgggGAGCCUGGUGgCccgggguggccacccUGCGcuuGGGGGc -3' miRNA: 3'- -CCGG----CUCGGACUAC-G---------------ACGCuu-CCUCC- -5' |
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2814 | 5' | -57 | NC_001491.2 | + | 148327 | 0.68 | 0.761831 |
Target: 5'- aGuCCGGGCCcgaagagGGUGCUG-GAGGuGAGGa -3' miRNA: 3'- cC-GGCUCGGa------CUACGACgCUUC-CUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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