Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2814 | 5' | -57 | NC_001491.2 | + | 144673 | 0.7 | 0.663513 |
Target: 5'- cGGCCGcGCUg---GCUGCGcggauGAGGGGGu -3' miRNA: 3'- -CCGGCuCGGacuaCGACGC-----UUCCUCC- -5' |
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2814 | 5' | -57 | NC_001491.2 | + | 1303 | 0.72 | 0.523151 |
Target: 5'- gGGCUGGGCCccacgcugGCUGCGggGGgagauGGGg -3' miRNA: 3'- -CCGGCUCGGacua----CGACGCuuCC-----UCC- -5' |
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2814 | 5' | -57 | NC_001491.2 | + | 139260 | 0.73 | 0.494292 |
Target: 5'- aGGCCGAGCg-GggGgaGCGGuagGGGAGGc -3' miRNA: 3'- -CCGGCUCGgaCuaCgaCGCU---UCCUCC- -5' |
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2814 | 5' | -57 | NC_001491.2 | + | 114464 | 0.76 | 0.362984 |
Target: 5'- aGGCCGAGCCccUGGUGCUGCccaucaccGAGGc -3' miRNA: 3'- -CCGGCUCGG--ACUACGACGcuuc----CUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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