Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28140 | 5' | -53.6 | NC_005887.1 | + | 36364 | 0.67 | 0.653153 |
|
Target: 5'- --cCGCCGcGCAGAUCGgccaaccGGACGAgUCGa -3' miRNA: 3'- uuaGCGGUuCGUCUAGC-------UCUGCU-GGC- -5' |
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| 28140 | 5' | -53.6 | NC_005887.1 | + | 38328 | 0.69 | 0.55228 |
|
Target: 5'- cGUCGCCAcugacaacAGCGugucGAUCGAGcgcuCGACCa -3' miRNA: 3'- uUAGCGGU--------UCGU----CUAGCUCu---GCUGGc -5' |
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| 28140 | 5' | -53.6 | NC_005887.1 | + | 40013 | 0.7 | 0.493249 |
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Target: 5'- cGUCGCgGugcgcaucauggcGCGGcUCGAGGCGGCCGg -3' miRNA: 3'- uUAGCGgUu------------CGUCuAGCUCUGCUGGC- -5' |
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| 28140 | 5' | -53.6 | NC_005887.1 | + | 40195 | 0.66 | 0.732742 |
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Target: 5'- gAAUCGCCGccGGcCAGGUCGAGGuCuucCCGa -3' miRNA: 3'- -UUAGCGGU--UC-GUCUAGCUCU-Gcu-GGC- -5' |
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| 28140 | 5' | -53.6 | NC_005887.1 | + | 40735 | 0.71 | 0.435275 |
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Target: 5'- aGGUCGCCGccCGcGUCGAGGCGAUCGc -3' miRNA: 3'- -UUAGCGGUucGUcUAGCUCUGCUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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