Results 41 - 45 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28140 | 5' | -53.6 | NC_005887.1 | + | 31341 | 0.66 | 0.732742 |
Target: 5'- -cUCGaCgAGGC-GAUCGAcGCGGCCGa -3' miRNA: 3'- uuAGC-GgUUCGuCUAGCUcUGCUGGC- -5' |
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28140 | 5' | -53.6 | NC_005887.1 | + | 973 | 0.66 | 0.71068 |
Target: 5'- gGGUCGCgAcGCGGAUCGAaGCcgaGGCCGc -3' miRNA: 3'- -UUAGCGgUuCGUCUAGCUcUG---CUGGC- -5' |
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28140 | 5' | -53.6 | NC_005887.1 | + | 21558 | 0.66 | 0.699518 |
Target: 5'- cGAUUGCCGgcGGCgaugcgagauAGGUCGAGAaaccCGACCc -3' miRNA: 3'- -UUAGCGGU--UCG----------UCUAGCUCU----GCUGGc -5' |
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28140 | 5' | -53.6 | NC_005887.1 | + | 4938 | 0.67 | 0.665661 |
Target: 5'- cAUCGUCGcgAGCGGAUCGAu-CGugCGc -3' miRNA: 3'- uUAGCGGU--UCGUCUAGCUcuGCugGC- -5' |
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28140 | 5' | -53.6 | NC_005887.1 | + | 26304 | 0.75 | 0.26465 |
Target: 5'- aGAUCGUCGAGCGGAUCG-GAccCGugCGc -3' miRNA: 3'- -UUAGCGGUUCGUCUAGCuCU--GCugGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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