Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28140 | 5' | -53.6 | NC_005887.1 | + | 4518 | 0.67 | 0.675865 |
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Target: 5'- -cUCGCCGacuacGGCGGcgucccgGUCGAGGCGAUg- -3' miRNA: 3'- uuAGCGGU-----UCGUC-------UAGCUCUGCUGgc -5' |
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| 28140 | 5' | -53.6 | NC_005887.1 | + | 15587 | 0.67 | 0.665661 |
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Target: 5'- uGAUCGCCGAagcugagacGCAGcgcacgggcGUCGcgcagcAGGCGGCCGg -3' miRNA: 3'- -UUAGCGGUU---------CGUC---------UAGC------UCUGCUGGC- -5' |
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| 28140 | 5' | -53.6 | NC_005887.1 | + | 24997 | 0.67 | 0.654292 |
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Target: 5'- uAGUUGCCGAGCgucAGcUCGcgccGGCGGCCGc -3' miRNA: 3'- -UUAGCGGUUCG---UCuAGCu---CUGCUGGC- -5' |
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| 28140 | 5' | -53.6 | NC_005887.1 | + | 14727 | 0.67 | 0.631499 |
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Target: 5'- cGAUCGCgCAGGCGacGAUCGAGaaaGCGAUUc -3' miRNA: 3'- -UUAGCG-GUUCGU--CUAGCUC---UGCUGGc -5' |
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| 28140 | 5' | -53.6 | NC_005887.1 | + | 16603 | 1.07 | 0.001354 |
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Target: 5'- aAAUCGCCAAGCAGAUCGAGACGACCGa -3' miRNA: 3'- -UUAGCGGUUCGUCUAGCUCUGCUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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