Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 28141 | 5' | -51.9 | NC_005887.1 | + | 3586 | 0.72 | 0.450241 |
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Target: 5'- aCGCACGUGCAGUgggagcucacguuGCGccagGCCGCgCAGcgCu -3' miRNA: 3'- -GCGUGUACGUCA-------------UGU----UGGCG-GUCaaG- -5' |
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| 28141 | 5' | -51.9 | NC_005887.1 | + | 29751 | 0.72 | 0.472312 |
|
Target: 5'- uGCGCGUGCAGcugccaaGCAAgCGCCAGc-- -3' miRNA: 3'- gCGUGUACGUCa------UGUUgGCGGUCaag -5' |
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| 28141 | 5' | -51.9 | NC_005887.1 | + | 38604 | 0.73 | 0.420723 |
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Target: 5'- aGCGCgAUGUAGcGCuuCgGCCAGUUCg -3' miRNA: 3'- gCGUG-UACGUCaUGuuGgCGGUCAAG- -5' |
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| 28141 | 5' | -51.9 | NC_005887.1 | + | 26498 | 0.73 | 0.406897 |
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Target: 5'- uCGCGCGgcUGCAGcgcgcguucccgcGCGGCCGCCAGcUCg -3' miRNA: 3'- -GCGUGU--ACGUCa------------UGUUGGCGGUCaAG- -5' |
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| 28141 | 5' | -51.9 | NC_005887.1 | + | 32673 | 0.74 | 0.340485 |
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Target: 5'- uCGCGCGUGCGcgcuucggcaaccgcGUACAgcgcacggauaGCCGCgCGGUUCa -3' miRNA: 3'- -GCGUGUACGU---------------CAUGU-----------UGGCG-GUCAAG- -5' |
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| 28141 | 5' | -51.9 | NC_005887.1 | + | 17351 | 1.11 | 0.000993 |
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Target: 5'- gCGCACAUGCAGUACAACCGCCAGUUCg -3' miRNA: 3'- -GCGUGUACGUCAUGUUGGCGGUCAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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