Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28141 | 5' | -51.9 | NC_005887.1 | + | 39605 | 0.69 | 0.641579 |
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Target: 5'- uCGCGCGgauUGgGGUACAgaGCCggcGCCAGUUg -3' miRNA: 3'- -GCGUGU---ACgUCAUGU--UGG---CGGUCAAg -5' |
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| 28141 | 5' | -51.9 | NC_005887.1 | + | 40578 | 0.67 | 0.765819 |
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Target: 5'- gCGCGCucgaGCaAGUACAACC-CgCGGUUCc -3' miRNA: 3'- -GCGUGua--CG-UCAUGUUGGcG-GUCAAG- -5' |
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| 28141 | 5' | -51.9 | NC_005887.1 | + | 40953 | 0.66 | 0.807351 |
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Target: 5'- aGCGCAUGCu---CGACCGCUucGGcgCg -3' miRNA: 3'- gCGUGUACGucauGUUGGCGG--UCaaG- -5' |
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| 28141 | 5' | -51.9 | NC_005887.1 | + | 41287 | 0.68 | 0.687777 |
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Target: 5'- gCGCACGggcGCGcUGCuGCCGCCGGg-- -3' miRNA: 3'- -GCGUGUa--CGUcAUGuUGGCGGUCaag -5' |
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| 28141 | 5' | -51.9 | NC_005887.1 | + | 41915 | 0.66 | 0.797243 |
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Target: 5'- gGCACGaGCAucagGC-GCCGCCAGaUCg -3' miRNA: 3'- gCGUGUaCGUca--UGuUGGCGGUCaAG- -5' |
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| 28141 | 5' | -51.9 | NC_005887.1 | + | 42261 | 0.67 | 0.744084 |
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Target: 5'- gCGCGCA-GCAaUGCGACgaGCCAGcgCg -3' miRNA: 3'- -GCGUGUaCGUcAUGUUGg-CGGUCaaG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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