Results 81 - 89 of 89 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28152 | 3' | -64.5 | NC_005887.1 | + | 36422 | 0.66 | 0.243113 |
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Target: 5'- gCGCaCCGCcuucCGCgCGGCCGGCuucuUGCc -3' miRNA: 3'- -GCG-GGCGua--GCG-GCCGGCCGuc--ACGc -5' |
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| 28152 | 3' | -64.5 | NC_005887.1 | + | 40264 | 0.66 | 0.249217 |
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Target: 5'- uGUUCGUGcugcCGCUGGCCGGCaagucGGUGCc -3' miRNA: 3'- gCGGGCGUa---GCGGCCGGCCG-----UCACGc -5' |
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| 28152 | 3' | -64.5 | NC_005887.1 | + | 538 | 0.66 | 0.249217 |
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Target: 5'- uCGgCCGCcgcgCGCCGcGCCGGauaCAGcGCGa -3' miRNA: 3'- -GCgGGCGua--GCGGC-CGGCC---GUCaCGC- -5' |
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| 28152 | 3' | -64.5 | NC_005887.1 | + | 29566 | 0.66 | 0.249217 |
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Target: 5'- gCGCCCGcCGUCgagGCC-GCCGGCuacgacaucGUGCu -3' miRNA: 3'- -GCGGGC-GUAG---CGGcCGGCCGu--------CACGc -5' |
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| 28152 | 3' | -64.5 | NC_005887.1 | + | 2779 | 0.66 | 0.249217 |
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Target: 5'- aGUCCGuCGUCGCgCGGCuCGGCcgcuUGCc -3' miRNA: 3'- gCGGGC-GUAGCG-GCCG-GCCGuc--ACGc -5' |
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| 28152 | 3' | -64.5 | NC_005887.1 | + | 11706 | 0.66 | 0.249217 |
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Target: 5'- gCGUCUGCAacgCGUCGaGCuCGGCAuUGCGc -3' miRNA: 3'- -GCGGGCGUa--GCGGC-CG-GCCGUcACGC- -5' |
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| 28152 | 3' | -64.5 | NC_005887.1 | + | 33447 | 0.66 | 0.255446 |
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Target: 5'- aGCCU-CAUCcuGCCcGCCGGCGcGUGCa -3' miRNA: 3'- gCGGGcGUAG--CGGcCGGCCGU-CACGc -5' |
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| 28152 | 3' | -64.5 | NC_005887.1 | + | 4288 | 0.66 | 0.255446 |
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Target: 5'- uCGCgCGCGaggucgacauccUCGCCgaGGCCGGcCAGcUGCc -3' miRNA: 3'- -GCGgGCGU------------AGCGG--CCGGCC-GUC-ACGc -5' |
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| 28152 | 3' | -64.5 | NC_005887.1 | + | 2328 | 0.66 | 0.255446 |
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Target: 5'- uCGCUgGCAUCuaUGGCgaucCGGCGG-GCGa -3' miRNA: 3'- -GCGGgCGUAGcgGCCG----GCCGUCaCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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