Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28152 | 5' | -52.8 | NC_005887.1 | + | 23654 | 0.71 | 0.482044 |
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Target: 5'- uACGCGCUGAUUGUcggagauaAUCCGGCGc--- -3' miRNA: 3'- cUGCGCGACUGGCA--------UAGGCUGCuuuu -5' |
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| 28152 | 5' | -52.8 | NC_005887.1 | + | 26438 | 0.69 | 0.581451 |
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Target: 5'- cGCGCGCUGcaGCCGcgcgagAUCCGACuuGAAg -3' miRNA: 3'- cUGCGCGAC--UGGCa-----UAGGCUGcuUUU- -5' |
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| 28152 | 5' | -52.8 | NC_005887.1 | + | 27422 | 0.69 | 0.581451 |
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Target: 5'- aGCGUGCUGACCGUcuugCCGAgGc--- -3' miRNA: 3'- cUGCGCGACUGGCAua--GGCUgCuuuu -5' |
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| 28152 | 5' | -52.8 | NC_005887.1 | + | 30269 | 0.73 | 0.382239 |
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Target: 5'- cGACGCgGCgUGGCUGg--CCGGCGAGAAg -3' miRNA: 3'- -CUGCG-CG-ACUGGCauaGGCUGCUUUU- -5' |
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| 28152 | 5' | -52.8 | NC_005887.1 | + | 32312 | 0.66 | 0.779616 |
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Target: 5'- cGGCGCGCcgcaGugCGUAggaccacacgccgaUgCCGGCGAAGAa -3' miRNA: 3'- -CUGCGCGa---CugGCAU--------------A-GGCUGCUUUU- -5' |
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| 28152 | 5' | -52.8 | NC_005887.1 | + | 32363 | 0.71 | 0.440512 |
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Target: 5'- cGGCGCGCcGGCUGgc-CCGACGAu-- -3' miRNA: 3'- -CUGCGCGaCUGGCauaGGCUGCUuuu -5' |
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| 28152 | 5' | -52.8 | NC_005887.1 | + | 33799 | 0.68 | 0.627174 |
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Target: 5'- cGGCGCGCUGAUCcUcgCCG-CGAu-- -3' miRNA: 3'- -CUGCGCGACUGGcAuaGGCuGCUuuu -5' |
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| 28152 | 5' | -52.8 | NC_005887.1 | + | 41133 | 0.66 | 0.772301 |
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Target: 5'- aGCGCGCgGAUCGUGUCCuuCGc--- -3' miRNA: 3'- cUGCGCGaCUGGCAUAGGcuGCuuuu -5' |
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| 28152 | 5' | -52.8 | NC_005887.1 | + | 41408 | 0.68 | 0.650106 |
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Target: 5'- cGGCGCGCgGAUCGUAcucaaCCGGCGu--- -3' miRNA: 3'- -CUGCGCGaCUGGCAUa----GGCUGCuuuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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