Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28153 | 3' | -53.2 | NC_005887.1 | + | 4245 | 0.66 | 0.774978 |
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Target: 5'- --cGGUCGCGGUCaUGUCGCcgcugucGACg -3' miRNA: 3'- gacUCAGCGCCAGcAUAGCGcua----CUG- -5' |
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| 28153 | 3' | -53.2 | NC_005887.1 | + | 7325 | 0.66 | 0.764588 |
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Target: 5'- -cGAuGUCGCGGUCGcUGcCGagguCGAUGAUa -3' miRNA: 3'- gaCU-CAGCGCCAGC-AUaGC----GCUACUG- -5' |
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| 28153 | 3' | -53.2 | NC_005887.1 | + | 15523 | 0.66 | 0.754054 |
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Target: 5'- uCUuGGUgCGCGGUC--AUCGCGgcGACg -3' miRNA: 3'- -GAcUCA-GCGCCAGcaUAGCGCuaCUG- -5' |
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| 28153 | 3' | -53.2 | NC_005887.1 | + | 33077 | 0.66 | 0.754054 |
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Target: 5'- uUGAuuaUCGaCGGcagCGUGUCGCGcAUGACc -3' miRNA: 3'- gACUc--AGC-GCCa--GCAUAGCGC-UACUG- -5' |
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| 28153 | 3' | -53.2 | NC_005887.1 | + | 35730 | 0.67 | 0.710733 |
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Target: 5'- gCUcGGUCGCGGUCGUGgcccacUCGCuc-GGCu -3' miRNA: 3'- -GAcUCAGCGCCAGCAU------AGCGcuaCUG- -5' |
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| 28153 | 3' | -53.2 | NC_005887.1 | + | 3779 | 0.67 | 0.710733 |
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Target: 5'- -cGGaUCGCGGUCGgcgCGCGGcucGACg -3' miRNA: 3'- gaCUcAGCGCCAGCauaGCGCUa--CUG- -5' |
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| 28153 | 3' | -53.2 | NC_005887.1 | + | 15621 | 0.68 | 0.662728 |
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Target: 5'- -cGAG-CGCGGUCGcg-CGCGccgagucgucgcccGUGACg -3' miRNA: 3'- gaCUCaGCGCCAGCauaGCGC--------------UACUG- -5' |
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| 28153 | 3' | -53.2 | NC_005887.1 | + | 15082 | 0.71 | 0.499334 |
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Target: 5'- -gGAGcCGcCGG-CGUGUCGCaGAUGAUg -3' miRNA: 3'- gaCUCaGC-GCCaGCAUAGCG-CUACUG- -5' |
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| 28153 | 3' | -53.2 | NC_005887.1 | + | 23912 | 1.11 | 0.000993 |
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Target: 5'- gCUGAGUCGCGGUCGUAUCGCGAUGACg -3' miRNA: 3'- -GACUCAGCGCCAGCAUAGCGCUACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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