Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28164 | 3' | -52.9 | NC_005887.1 | + | 4688 | 0.66 | 0.753077 |
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Target: 5'- gGCGAGggucuGAUGgGCGCGCCgAUc- -3' miRNA: 3'- aUGCUUaac--CUACgCGCGCGGgUAac -5' |
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| 28164 | 3' | -52.9 | NC_005887.1 | + | 13542 | 0.66 | 0.742156 |
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Target: 5'- cGCGAAUgcgaucgGcGAUGCGCuGCcGCCCGa-- -3' miRNA: 3'- aUGCUUAa------C-CUACGCG-CG-CGGGUaac -5' |
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| 28164 | 3' | -52.9 | NC_005887.1 | + | 16192 | 0.66 | 0.731113 |
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Target: 5'- gGCGGggUGGuUGCGCGCGgCgCAg-- -3' miRNA: 3'- aUGCUuaACCuACGCGCGCgG-GUaac -5' |
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| 28164 | 3' | -52.9 | NC_005887.1 | + | 6079 | 0.66 | 0.719961 |
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Target: 5'- aACGAA---GAUGCGCGCGCUg---- -3' miRNA: 3'- aUGCUUaacCUACGCGCGCGGguaac -5' |
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| 28164 | 3' | -52.9 | NC_005887.1 | + | 4564 | 0.66 | 0.719961 |
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Target: 5'- gGCGAGcagacgcGcGAUGCGCGCGCCg---- -3' miRNA: 3'- aUGCUUaa-----C-CUACGCGCGCGGguaac -5' |
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| 28164 | 3' | -52.9 | NC_005887.1 | + | 12083 | 0.66 | 0.719961 |
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Target: 5'- uUGCGAGcgGcGUGCGUGCGCCgGa-- -3' miRNA: 3'- -AUGCUUaaCcUACGCGCGCGGgUaac -5' |
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| 28164 | 3' | -52.9 | NC_005887.1 | + | 17036 | 0.66 | 0.708712 |
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Target: 5'- aACGGAUcGGAUGCGCaGCGgCaCGcUUGg -3' miRNA: 3'- aUGCUUAaCCUACGCG-CGCgG-GU-AAC- -5' |
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| 28164 | 3' | -52.9 | NC_005887.1 | + | 4361 | 0.67 | 0.697381 |
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Target: 5'- cGCGGAUgUGGAUGuCGaguaCGacaGCCCGUUGa -3' miRNA: 3'- aUGCUUA-ACCUAC-GC----GCg--CGGGUAAC- -5' |
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| 28164 | 3' | -52.9 | NC_005887.1 | + | 39243 | 0.67 | 0.651486 |
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Target: 5'- gACGuguucgGGAUcucGCGCGCGUCCGUc- -3' miRNA: 3'- aUGCuuaa--CCUA---CGCGCGCGGGUAac -5' |
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| 28164 | 3' | -52.9 | NC_005887.1 | + | 28908 | 1.06 | 0.00173 |
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Target: 5'- cUACGAAUUGGAUGCGCGCGCCCAUUGc -3' miRNA: 3'- -AUGCUUAACCUACGCGCGCGGGUAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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