Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
28166 | 5' | -53.9 | NC_005887.1 | + | 35217 | 0.66 | 0.703148 |
Target: 5'- --uGCGCAgcaGCUUGUCGGCCuGCg- -3' miRNA: 3'- gacCGCGUacaUGAACAGCUGG-CGaa -5' |
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28166 | 5' | -53.9 | NC_005887.1 | + | 8379 | 0.66 | 0.703147 |
Target: 5'- -cGGCGCcgGUGCUgacgGaCG-CCGCa- -3' miRNA: 3'- gaCCGCGuaCAUGAa---CaGCuGGCGaa -5' |
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28166 | 5' | -53.9 | NC_005887.1 | + | 454 | 0.67 | 0.587711 |
Target: 5'- uCUGGCGCGUGUggugcgggcaugaGCaaGcUGACCGCa- -3' miRNA: 3'- -GACCGCGUACA-------------UGaaCaGCUGGCGaa -5' |
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28166 | 5' | -53.9 | NC_005887.1 | + | 24999 | 0.68 | 0.53249 |
Target: 5'- aCUGGCGguUGUACUgcaUGugCGCg- -3' miRNA: 3'- -GACCGCguACAUGAacaGCugGCGaa -5' |
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28166 | 5' | -53.9 | NC_005887.1 | + | 1118 | 0.75 | 0.218926 |
Target: 5'- -aGGUGCGUGUGCUUGUCGA--GCUc -3' miRNA: 3'- gaCCGCGUACAUGAACAGCUggCGAa -5' |
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28166 | 5' | -53.9 | NC_005887.1 | + | 30082 | 1.05 | 0.001619 |
Target: 5'- gCUGGCGCAUGUACUUGUCGACCGCUUc -3' miRNA: 3'- -GACCGCGUACAUGAACAGCUGGCGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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