Results 41 - 48 of 48 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28181 | 5' | -63.4 | NC_005887.1 | + | 29738 | 0.72 | 0.099344 |
Target: 5'- -cGCGCCGCGC-GUGCAcGGUcuugcccggCGAGCUg -3' miRNA: 3'- acCGCGGCGCGcCACGU-CCA---------GCUCGA- -5' |
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28181 | 5' | -63.4 | NC_005887.1 | + | 21089 | 0.72 | 0.096637 |
Target: 5'- cGcCGCCGcCGCGGUGCccccgaGGGUCGAuGCg -3' miRNA: 3'- aCcGCGGC-GCGCCACG------UCCAGCU-CGa -5' |
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28181 | 5' | -63.4 | NC_005887.1 | + | 36975 | 0.73 | 0.077366 |
Target: 5'- cGGCGUCGCGCGGgucgagcucGCcGG-CGAGCa -3' miRNA: 3'- aCCGCGGCGCGCCa--------CGuCCaGCUCGa -5' |
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28181 | 5' | -63.4 | NC_005887.1 | + | 455 | 0.74 | 0.06725 |
Target: 5'- cUGGCG-CGUGUGGUGCGGGcaUGAGCa -3' miRNA: 3'- -ACCGCgGCGCGCCACGUCCa-GCUCGa -5' |
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28181 | 5' | -63.4 | NC_005887.1 | + | 26657 | 0.75 | 0.050709 |
Target: 5'- cGGCggaaucauccgGCCGCGCGGcggugaGCuGGUCGAGCUc -3' miRNA: 3'- aCCG-----------CGGCGCGCCa-----CGuCCAGCUCGA- -5' |
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28181 | 5' | -63.4 | NC_005887.1 | + | 26435 | 0.78 | 0.034033 |
Target: 5'- cGGC-CCGCGCGGaacGCAGGUCGgccAGCUg -3' miRNA: 3'- aCCGcGGCGCGCCa--CGUCCAGC---UCGA- -5' |
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28181 | 5' | -63.4 | NC_005887.1 | + | 12058 | 0.78 | 0.031232 |
Target: 5'- aGGCGCCGCGCuGcaGCAGGUCG-GCa -3' miRNA: 3'- aCCGCGGCGCGcCa-CGUCCAGCuCGa -5' |
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28181 | 5' | -63.4 | NC_005887.1 | + | 41032 | 1.06 | 0.000179 |
Target: 5'- cUGGCGCCGCGCGGUGCAGGUCGAGCUc -3' miRNA: 3'- -ACCGCGGCGCGCCACGUCCAGCUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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