miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
28194 3' -49.3 NC_005891.1 + 22519 0.66 0.934926
Target:  5'- -uUCCGCCUGUUUCgAUUCGagugaACAUaccgugAGGa -3'
miRNA:   3'- guAGGCGGGCAAAG-UAGGU-----UGUA------UCC- -5'
28194 3' -49.3 NC_005891.1 + 10933 0.66 0.934926
Target:  5'- gCcgCCGCCCGUUagcUCGguUCCGAUAg--- -3'
miRNA:   3'- -GuaGGCGGGCAA---AGU--AGGUUGUaucc -5'
28194 3' -49.3 NC_005891.1 + 23914 0.66 0.92301
Target:  5'- --aCUGCUCGcugUCAUCCGAUucguGUGGGc -3'
miRNA:   3'- guaGGCGGGCaa-AGUAGGUUG----UAUCC- -5'
28194 3' -49.3 NC_005891.1 + 12862 0.73 0.581411
Target:  5'- cCAUCCGUCCGcucUCuUCCAACAgugcgAGGc -3'
miRNA:   3'- -GUAGGCGGGCaa-AGuAGGUUGUa----UCC- -5'
28194 3' -49.3 NC_005891.1 + 1806 1.12 0.001894
Target:  5'- cCAUCCGCCCGUUUCAUCCAACAUAGGa -3'
miRNA:   3'- -GUAGGCGGGCAAAGUAGGUUGUAUCC- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.