miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
28199 5' -50.4 NC_005891.1 + 11482 0.71 0.525683
Target:  5'- -uGGUGgcGCAGGcGGCAacGGCUCCGGAg -3'
miRNA:   3'- acUUACauUGUCC-UCGU--CCGAGGUUU- -5'
28199 5' -50.4 NC_005891.1 + 16232 0.68 0.703423
Target:  5'- cUGggUGUGGCAGGuaguuuuGCAGGUgcguucgcuucCCAAAc -3'
miRNA:   3'- -ACuuACAUUGUCCu------CGUCCGa----------GGUUU- -5'
28199 5' -50.4 NC_005891.1 + 14793 0.66 0.824271
Target:  5'- ----cGUuGCAGGAGCAGGaagCCGu- -3'
miRNA:   3'- acuuaCAuUGUCCUCGUCCga-GGUuu -5'
28199 5' -50.4 NC_005891.1 + 12463 0.67 0.793411
Target:  5'- uUGGAcuUGUuGCGGGAGCAGaaaauaacuuGCUCCu-- -3'
miRNA:   3'- -ACUU--ACAuUGUCCUCGUC----------CGAGGuuu -5'
28199 5' -50.4 NC_005891.1 + 10594 1.07 0.002057
Target:  5'- gUGAAUGUAACAGGAGCAGGCUCCAAAg -3'
miRNA:   3'- -ACUUACAUUGUCCUCGUCCGAGGUUU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.