Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28200 | 3' | -52.8 | NC_005891.1 | + | 13430 | 0.69 | 0.533053 |
Target: 5'- -cGGcugaaUUGCGGUggUUGGCCACCcaagaagaAGUgCGCa -3' miRNA: 3'- uaCC-----AACGCCA--AACCGGUGG--------UUA-GCG- -5' |
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28200 | 3' | -52.8 | NC_005891.1 | + | 20507 | 0.68 | 0.601531 |
Target: 5'- -cGGUguUGgGGUUaaggUGGCCGCCcucccugaGGUCGUa -3' miRNA: 3'- uaCCA--ACgCCAA----ACCGGUGG--------UUAGCG- -5' |
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28200 | 3' | -52.8 | NC_005891.1 | + | 39430 | 0.7 | 0.488961 |
Target: 5'- aGUGGggGCGGa--GGCCgauGCCAAgggggCGCa -3' miRNA: 3'- -UACCaaCGCCaaaCCGG---UGGUUa----GCG- -5' |
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28200 | 3' | -52.8 | NC_005891.1 | + | 11002 | 1.11 | 0.000717 |
Target: 5'- gAUGGUUGCGGUUUGGCCACCAAUCGCa -3' miRNA: 3'- -UACCAACGCCAAACCGGUGGUUAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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