miRNA: 3'- uUUCCUGUCUUUGGuuacGGCG-UGGc -5'
miRNA: 3'- uUUCCUGUCUUUGGUuacGGCGUggC- -5'
miRNA: 3'- uuUCCUGUCUUUGGUUACGG---------CGUggc -5'
miRNA: 3'- uUUCCUGUCUUUGGUUACGG------CGUG-GC- -5'
miRNA: 3'- -UUUCCUGUCUUUGGUUACGGCGUGGC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28200 | 5' | -51.7 | NC_005891.1 | + | 5966 | 0.66 | 0.768642 |
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Target: 5'- uGAGGACGGAGGCUu---CCGCuACCc -3' miRNA: 3'- uUUCCUGUCUUUGGuuacGGCG-UGGc -5' |
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| 28200 | 5' | -51.7 | NC_005891.1 | + | 30994 | 0.7 | 0.573318 |
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Target: 5'- cGAGGAUAGAAACCA---CCGCAaaGg -3' miRNA: 3'- uUUCCUGUCUUUGGUuacGGCGUggC- -5' |
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| 28200 | 5' | -51.7 | NC_005891.1 | + | 16213 | 0.72 | 0.451162 |
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Target: 5'- -cGGGACAGAGGCCAAUGCUcugggugugGCAg-- -3' miRNA: 3'- uuUCCUGUCUUUGGUUACGG---------CGUggc -5' |
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| 28200 | 5' | -51.7 | NC_005891.1 | + | 39428 | 0.8 | 0.145659 |
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Target: 5'- uGGGGGCGGAGGCCGAUGCCaaggggGCGCaCGa -3' miRNA: 3'- uUUCCUGUCUUUGGUUACGG------CGUG-GC- -5' |
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| 28200 | 5' | -51.7 | NC_005891.1 | + | 10968 | 1.06 | 0.002001 |
|
Target: 5'- gAAAGGACAGAAACCAAUGCCGCACCGc -3' miRNA: 3'- -UUUCCUGUCUUUGGUUACGGCGUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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