Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28202 | 3' | -46.4 | NC_005891.1 | + | 22959 | 0.68 | 0.943549 |
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Target: 5'- gCgGCguGGUgGAUUGAuGAACAUGGCa -3' miRNA: 3'- gGgUGguUCA-CUAACU-UUUGUACCGg -5' |
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| 28202 | 3' | -46.4 | NC_005891.1 | + | 29986 | 0.69 | 0.932363 |
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Target: 5'- -aCGCCAAGUacAUUGAAGgcgcggaagaagGCAUGGCUu -3' miRNA: 3'- ggGUGGUUCAc-UAACUUU------------UGUACCGG- -5' |
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| 28202 | 3' | -46.4 | NC_005891.1 | + | 8579 | 0.7 | 0.891027 |
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Target: 5'- uUCAaaAAGUacgGGUUGAAGGCAUGGCg -3' miRNA: 3'- gGGUggUUCA---CUAACUUUUGUACCGg -5' |
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| 28202 | 3' | -46.4 | NC_005891.1 | + | 30779 | 0.71 | 0.874725 |
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Target: 5'- aCCCACCAcAG-GAgcGAAAACAUGaGUg -3' miRNA: 3'- -GGGUGGU-UCaCUaaCUUUUGUAC-CGg -5' |
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| 28202 | 3' | -46.4 | NC_005891.1 | + | 8917 | 0.72 | 0.819031 |
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Target: 5'- uCCCGCUGAGgGAcUGAAGuUAUGGUCg -3' miRNA: 3'- -GGGUGGUUCaCUaACUUUuGUACCGG- -5' |
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| 28202 | 3' | -46.4 | NC_005891.1 | + | 18917 | 0.76 | 0.578624 |
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Target: 5'- aCC-CUAAGcuuugGAUUGAAGGCAUGGCa -3' miRNA: 3'- gGGuGGUUCa----CUAACUUUUGUACCGg -5' |
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| 28202 | 3' | -46.4 | NC_005891.1 | + | 23533 | 1.17 | 0.001863 |
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Target: 5'- gCCCACCAAGUGAUUGAAAACAUGGCCa -3' miRNA: 3'- -GGGUGGUUCACUAACUUUUGUACCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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