Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28203 | 5' | -52.6 | NC_005891.1 | + | 1093 | 0.77 | 0.207112 |
Target: 5'- gUGUCGACGUCGACACUauuccCGAUGCAg -3' miRNA: 3'- -ACAGCUGCAGCUGUGGgu---GUUGUGUg -5' |
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28203 | 5' | -52.6 | NC_005891.1 | + | 1147 | 0.66 | 0.76533 |
Target: 5'- gUGUCGACGUCGACACagguuuCGAUu--- -3' miRNA: 3'- -ACAGCUGCAGCUGUGggu---GUUGugug -5' |
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28203 | 5' | -52.6 | NC_005891.1 | + | 7047 | 0.72 | 0.425131 |
Target: 5'- gUGUCGAUGUCGACACaCCcUAAU-CACc -3' miRNA: 3'- -ACAGCUGCAGCUGUG-GGuGUUGuGUG- -5' |
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28203 | 5' | -52.6 | NC_005891.1 | + | 9011 | 0.72 | 0.445164 |
Target: 5'- gUGUCGAUGUCGACAuacuUCCGCu-UugGCg -3' miRNA: 3'- -ACAGCUGCAGCUGU----GGGUGuuGugUG- -5' |
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28203 | 5' | -52.6 | NC_005891.1 | + | 11841 | 0.66 | 0.76533 |
Target: 5'- aGUCGugGcUGcCACCCccagugaguggcGCAAUACGCu -3' miRNA: 3'- aCAGCugCaGCuGUGGG------------UGUUGUGUG- -5' |
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28203 | 5' | -52.6 | NC_005891.1 | + | 16836 | 0.75 | 0.301234 |
Target: 5'- gUGUCGACGUCGACACaCC-CGA-ACAg -3' miRNA: 3'- -ACAGCUGCAGCUGUG-GGuGUUgUGUg -5' |
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28203 | 5' | -52.6 | NC_005891.1 | + | 16889 | 0.81 | 0.120719 |
Target: 5'- gUGUCGACGUCGACACCCucccccuCAgGCu -3' miRNA: 3'- -ACAGCUGCAGCUGUGGGuguu---GUgUG- -5' |
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28203 | 5' | -52.6 | NC_005891.1 | + | 21750 | 0.82 | 0.107407 |
Target: 5'- gUGUCGACGUCGACACCCAUcuguuAUuuGCa -3' miRNA: 3'- -ACAGCUGCAGCUGUGGGUGu----UGugUG- -5' |
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28203 | 5' | -52.6 | NC_005891.1 | + | 21804 | 0.79 | 0.168424 |
Target: 5'- gUGUCGACGUCGACACCCuacugucccuuccCGACGUAUa -3' miRNA: 3'- -ACAGCUGCAGCUGUGGGu------------GUUGUGUG- -5' |
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28203 | 5' | -52.6 | NC_005891.1 | + | 22492 | 0.69 | 0.597412 |
Target: 5'- cGUCcACGUCGGgaACCCcCGACGCAg -3' miRNA: 3'- aCAGcUGCAGCUg-UGGGuGUUGUGUg -5' |
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28203 | 5' | -52.6 | NC_005891.1 | + | 24581 | 0.76 | 0.271359 |
Target: 5'- gUGUCGACGUCGACACCCu-------- -3' miRNA: 3'- -ACAGCUGCAGCUGUGGGuguugugug -5' |
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28203 | 5' | -52.6 | NC_005891.1 | + | 24635 | 1.11 | 0.000982 |
Target: 5'- gUGUCGACGUCGACACCCACAACACACa -3' miRNA: 3'- -ACAGCUGCAGCUGUGGGUGUUGUGUG- -5' |
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28203 | 5' | -52.6 | NC_005891.1 | + | 29282 | 0.76 | 0.271359 |
Target: 5'- gUGUCGACGUCGACACCCu-------- -3' miRNA: 3'- -ACAGCUGCAGCUGUGGGuguugugug -5' |
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28203 | 5' | -52.6 | NC_005891.1 | + | 29336 | 0.8 | 0.147758 |
Target: 5'- gUGUCGACGUCGACACCCuCuuuuuACugGa -3' miRNA: 3'- -ACAGCUGCAGCUGUGGGuGu----UGugUg -5' |
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28203 | 5' | -52.6 | NC_005891.1 | + | 33180 | 0.9 | 0.030742 |
Target: 5'- gUGUCGACGUCGACACCCaucauuucagcagcuACAGCAUAUa -3' miRNA: 3'- -ACAGCUGCAGCUGUGGG---------------UGUUGUGUG- -5' |
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28203 | 5' | -52.6 | NC_005891.1 | + | 33234 | 0.74 | 0.342059 |
Target: 5'- gUGUCGACGUCGACACaCUgguaGCGAaAUACc -3' miRNA: 3'- -ACAGCUGCAGCUGUG-GG----UGUUgUGUG- -5' |
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28203 | 5' | -52.6 | NC_005891.1 | + | 34710 | 0.8 | 0.131693 |
Target: 5'- gUGUCGACGUCGACACCCAUc------ -3' miRNA: 3'- -ACAGCUGCAGCUGUGGGUGuugugug -5' |
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28203 | 5' | -52.6 | NC_005891.1 | + | 34764 | 0.79 | 0.170338 |
Target: 5'- gUGUCGACGUCGACACCCuACuuaaggagcCAgACa -3' miRNA: 3'- -ACAGCUGCAGCUGUGGG-UGuu-------GUgUG- -5' |
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28203 | 5' | -52.6 | NC_005891.1 | + | 36171 | 0.73 | 0.368408 |
Target: 5'- gUGUCGACGUCGACACCgC-CuuUGCGa -3' miRNA: 3'- -ACAGCUGCAGCUGUGG-GuGuuGUGUg -5' |
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28203 | 5' | -52.6 | NC_005891.1 | + | 36225 | 0.76 | 0.237422 |
Target: 5'- gUGUCGACGUCGACACaCUGCcgaGCAUc -3' miRNA: 3'- -ACAGCUGCAGCUGUG-GGUGuugUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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