Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28205 | 5' | -49.5 | NC_005891.1 | + | 1263 | 0.67 | 0.863563 |
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Target: 5'- uGGCUAUCAagAGGa-AGCAUacgaccacaUCCUGUc -3' miRNA: 3'- -CCGAUAGUagUUCggUCGUA---------AGGACG- -5' |
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| 28205 | 5' | -49.5 | NC_005891.1 | + | 38637 | 0.67 | 0.845507 |
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Target: 5'- uGGCuUGUCAUCGGcuugcucgucGUCAGC-UUgCUGCg -3' miRNA: 3'- -CCG-AUAGUAGUU----------CGGUCGuAAgGACG- -5' |
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| 28205 | 5' | -49.5 | NC_005891.1 | + | 35617 | 0.68 | 0.806374 |
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Target: 5'- -aCUAcCAUCAAGUCAGgCucgccUUCCUGCu -3' miRNA: 3'- ccGAUaGUAGUUCGGUC-Gu----AAGGACG- -5' |
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| 28205 | 5' | -49.5 | NC_005891.1 | + | 34063 | 0.69 | 0.77476 |
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Target: 5'- aGGUcacgGUCAUCAAaguGCCAGCucaCUUGCc -3' miRNA: 3'- -CCGa---UAGUAGUU---CGGUCGuaaGGACG- -5' |
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| 28205 | 5' | -49.5 | NC_005891.1 | + | 1993 | 0.73 | 0.508626 |
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Target: 5'- uGGCcAUCAUUGAGCgUAGCcgUaCCUGCu -3' miRNA: 3'- -CCGaUAGUAGUUCG-GUCGuaA-GGACG- -5' |
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| 28205 | 5' | -49.5 | NC_005891.1 | + | 14050 | 0.74 | 0.475389 |
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Target: 5'- aGUUGUUcgCGAGC--GCAUUCCUGCa -3' miRNA: 3'- cCGAUAGuaGUUCGguCGUAAGGACG- -5' |
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| 28205 | 5' | -49.5 | NC_005891.1 | + | 27684 | 1.15 | 0.000932 |
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Target: 5'- cGGCUAUCAUCAAGCCAGCAUUCCUGCc -3' miRNA: 3'- -CCGAUAGUAGUUCGGUCGUAAGGACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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