Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2821 | 5' | -53 | NC_001491.2 | + | 107152 | 0.66 | 0.972635 |
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Target: 5'- cCGC-AUUGAccgugcuUAGUGggugGGGgUGGCCAg -3' miRNA: 3'- aGUGuUAGCU-------AUCACa---CCCgACCGGU- -5' |
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| 2821 | 5' | -53 | NC_001491.2 | + | 123218 | 0.67 | 0.950411 |
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Target: 5'- gCGCAG-CGGguccgccgAGUGgaagacgggcaugGGGCUGGCCGc -3' miRNA: 3'- aGUGUUaGCUa-------UCACa------------CCCGACCGGU- -5' |
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| 2821 | 5' | -53 | NC_001491.2 | + | 50968 | 0.67 | 0.947805 |
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Target: 5'- uUCAUc--CGAUGG-GU-GGCUGGCCAu -3' miRNA: 3'- -AGUGuuaGCUAUCaCAcCCGACCGGU- -5' |
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| 2821 | 5' | -53 | NC_001491.2 | + | 78150 | 0.67 | 0.940418 |
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Target: 5'- aUCGCugcUCGAUgugcuucaucauguaGGUGUGGGUgGGCUg -3' miRNA: 3'- -AGUGuu-AGCUA---------------UCACACCCGaCCGGu -5' |
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| 2821 | 5' | -53 | NC_001491.2 | + | 16467 | 0.69 | 0.884011 |
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Target: 5'- aUCAUAGUCGAUgucGGUGgaaaacgaUGGGUUGGUg- -3' miRNA: 3'- -AGUGUUAGCUA---UCAC--------ACCCGACCGgu -5' |
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| 2821 | 5' | -53 | NC_001491.2 | + | 144014 | 0.7 | 0.845356 |
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Target: 5'- -uGCGAUCGAUAGUccucgaaGGCUGGCUg -3' miRNA: 3'- agUGUUAGCUAUCAcac----CCGACCGGu -5' |
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| 2821 | 5' | -53 | NC_001491.2 | + | 57114 | 0.72 | 0.722598 |
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Target: 5'- aCGCAAaCGAUgaagcguGGUGUGGGCgcaugcaGGCCGa -3' miRNA: 3'- aGUGUUaGCUA-------UCACACCCGa------CCGGU- -5' |
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| 2821 | 5' | -53 | NC_001491.2 | + | 128326 | 0.76 | 0.495655 |
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Target: 5'- cCACGGcaCGcgGGUGUGGGCUGGCg- -3' miRNA: 3'- aGUGUUa-GCuaUCACACCCGACCGgu -5' |
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| 2821 | 5' | -53 | NC_001491.2 | + | 119339 | 1.09 | 0.00466 |
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Target: 5'- aUCACAAUCGAUAGUGUGGGCUGGCCAc -3' miRNA: 3'- -AGUGUUAGCUAUCACACCCGACCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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