miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
28214 3' -54.3 NC_005891.1 + 12301 0.66 0.648138
Target:  5'- -cCACUGGUGUGucggucacagcGGCG--ACGGCCAUa -3'
miRNA:   3'- uaGUGACUACGC-----------CUGCauUGCCGGUA- -5'
28214 3' -54.3 NC_005891.1 + 4630 0.66 0.625048
Target:  5'- -gCACUGGUugacccGCaGugGUggUGGCCAUa -3'
miRNA:   3'- uaGUGACUA------CGcCugCAuuGCCGGUA- -5'
28214 3' -54.3 NC_005891.1 + 13431 0.68 0.500692
Target:  5'- --gGCUGAauUGCGGugGU--UGGCCAc -3'
miRNA:   3'- uagUGACU--ACGCCugCAuuGCCGGUa -5'
28214 3' -54.3 NC_005891.1 + 12796 0.69 0.437466
Target:  5'- -gCACUGAUuggaagagaGCGGACGgAugGGUCGUg -3'
miRNA:   3'- uaGUGACUA---------CGCCUGCaUugCCGGUA- -5'
28214 3' -54.3 NC_005891.1 + 19375 0.7 0.407616
Target:  5'- -cCACUGGUaaCGGA-GUGACGGCCAc -3'
miRNA:   3'- uaGUGACUAc-GCCUgCAUUGCCGGUa -5'
28214 3' -54.3 NC_005891.1 + 4524 1.03 0.001728
Target:  5'- cAUCACUGAUGCGGACGUAACGGCCAUc -3'
miRNA:   3'- -UAGUGACUACGCCUGCAUUGCCGGUA- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.