Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28215 | 5' | -48.6 | NC_005891.1 | + | 39437 | 0.66 | 0.938479 |
Target: 5'- gGGGCGAAguGGgGGCGGAGGccgauGCCaaggGGGCg -3' miRNA: 3'- -CCUGUUU--UCaCCGUCUUC-----UGGg---UUCG- -5' |
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28215 | 5' | -48.6 | NC_005891.1 | + | 10838 | 0.66 | 0.926816 |
Target: 5'- cGGCGGcGGcGGCGGc-GGCUCAGGCg -3' miRNA: 3'- cCUGUUuUCaCCGUCuuCUGGGUUCG- -5' |
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28215 | 5' | -48.6 | NC_005891.1 | + | 28097 | 0.66 | 0.926816 |
Target: 5'- uGGAUGGc-GUGGUgaGGGAGAaCCAAGCc -3' miRNA: 3'- -CCUGUUuuCACCG--UCUUCUgGGUUCG- -5' |
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28215 | 5' | -48.6 | NC_005891.1 | + | 33023 | 0.67 | 0.913878 |
Target: 5'- -cGCGAAA---GCGGAAGACCCA-GCu -3' miRNA: 3'- ccUGUUUUcacCGUCUUCUGGGUuCG- -5' |
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28215 | 5' | -48.6 | NC_005891.1 | + | 6871 | 0.67 | 0.890556 |
Target: 5'- -uACAAGAGUGGUAGccAAGGCCauaucggucauAGCg -3' miRNA: 3'- ccUGUUUUCACCGUC--UUCUGGgu---------UCG- -5' |
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28215 | 5' | -48.6 | NC_005891.1 | + | 16520 | 0.68 | 0.884234 |
Target: 5'- gGGGCGAuAGGUGGaaucaAGAgcaaaaAGGCCgAGGCc -3' miRNA: 3'- -CCUGUU-UUCACCg----UCU------UCUGGgUUCG- -5' |
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28215 | 5' | -48.6 | NC_005891.1 | + | 38865 | 0.68 | 0.867602 |
Target: 5'- uGGACGccAGAGUGGUGGugagcaaacaGGGACaCgAAGCa -3' miRNA: 3'- -CCUGU--UUUCACCGUC----------UUCUG-GgUUCG- -5' |
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28215 | 5' | -48.6 | NC_005891.1 | + | 7527 | 0.68 | 0.858858 |
Target: 5'- gGGAC---GGUGGUAGAAGGaaacauguUCCAAGa -3' miRNA: 3'- -CCUGuuuUCACCGUCUUCU--------GGGUUCg -5' |
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28215 | 5' | -48.6 | NC_005891.1 | + | 15191 | 0.68 | 0.849841 |
Target: 5'- uGGGCAAcGGcGGCGGuguuuuGGCCguGGCg -3' miRNA: 3'- -CCUGUUuUCaCCGUCuu----CUGGguUCG- -5' |
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28215 | 5' | -48.6 | NC_005891.1 | + | 27757 | 0.69 | 0.831027 |
Target: 5'- gGGACGGGGacaGGCAGAuugucuuacacuGGACCCGcacugcAGCa -3' miRNA: 3'- -CCUGUUUUca-CCGUCU------------UCUGGGU------UCG- -5' |
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28215 | 5' | -48.6 | NC_005891.1 | + | 11066 | 0.7 | 0.758237 |
Target: 5'- uGGACGGGAGguaaaGGCGGAAGaagcggauaccGCUCAgacGGCa -3' miRNA: 3'- -CCUGUUUUCa----CCGUCUUC-----------UGGGU---UCG- -5' |
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28215 | 5' | -48.6 | NC_005891.1 | + | 30085 | 0.7 | 0.758237 |
Target: 5'- uGGAgCAAGGccGUGGUucAGAAGACgUAAGCu -3' miRNA: 3'- -CCU-GUUUU--CACCG--UCUUCUGgGUUCG- -5' |
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28215 | 5' | -48.6 | NC_005891.1 | + | 6842 | 1.16 | 0.00106 |
Target: 5'- uGGACAAAAGUGGCAGAAGACCCAAGCg -3' miRNA: 3'- -CCUGUUUUCACCGUCUUCUGGGUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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