miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
28218 3' -44.3 NC_005891.1 + 33781 0.66 0.996814
Target:  5'- --gGCgg-CGAGCAUUGACUCC-UCGc -3'
miRNA:   3'- cgaUGaagGCUUGUAAUUGAGGuAGU- -5'
28218 3' -44.3 NC_005891.1 + 14303 0.66 0.996814
Target:  5'- uUUGCUgUCUGcgUAUUGACUCCGUUu -3'
miRNA:   3'- cGAUGA-AGGCuuGUAAUUGAGGUAGu -5'
28218 3' -44.3 NC_005891.1 + 16474 0.67 0.992327
Target:  5'- uGCUGCUUuuGGugGggcuUUAuccGCUCUAUCGc -3'
miRNA:   3'- -CGAUGAAggCUugU----AAU---UGAGGUAGU- -5'
28218 3' -44.3 NC_005891.1 + 32829 0.67 0.991005
Target:  5'- cGCUuCUUCaCGAGCGUUAACagCAgacgCAa -3'
miRNA:   3'- -CGAuGAAG-GCUUGUAAUUGagGUa---GU- -5'
28218 3' -44.3 NC_005891.1 + 30014 0.68 0.985542
Target:  5'- aGCUuacguCUUCUGAACcacggccuuGCUCCAUCu -3'
miRNA:   3'- -CGAu----GAAGGCUUGuaau-----UGAGGUAGu -5'
28218 3' -44.3 NC_005891.1 + 13108 0.84 0.35085
Target:  5'- uUUGCUUCCGAACAUUGACUUUuUCAu -3'
miRNA:   3'- cGAUGAAGGCUUGUAAUUGAGGuAGU- -5'
28218 3' -44.3 NC_005891.1 + 13007 1.14 0.005222
Target:  5'- aGCUACUUCCGAACAUUAACUCCAUCAa -3'
miRNA:   3'- -CGAUGAAGGCUUGUAAUUGAGGUAGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.