Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28218 | 3' | -44.3 | NC_005891.1 | + | 33781 | 0.66 | 0.996814 |
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Target: 5'- --gGCgg-CGAGCAUUGACUCC-UCGc -3' miRNA: 3'- cgaUGaagGCUUGUAAUUGAGGuAGU- -5' |
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| 28218 | 3' | -44.3 | NC_005891.1 | + | 14303 | 0.66 | 0.996814 |
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Target: 5'- uUUGCUgUCUGcgUAUUGACUCCGUUu -3' miRNA: 3'- cGAUGA-AGGCuuGUAAUUGAGGUAGu -5' |
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| 28218 | 3' | -44.3 | NC_005891.1 | + | 16474 | 0.67 | 0.992327 |
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Target: 5'- uGCUGCUUuuGGugGggcuUUAuccGCUCUAUCGc -3' miRNA: 3'- -CGAUGAAggCUugU----AAU---UGAGGUAGU- -5' |
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| 28218 | 3' | -44.3 | NC_005891.1 | + | 32829 | 0.67 | 0.991005 |
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Target: 5'- cGCUuCUUCaCGAGCGUUAACagCAgacgCAa -3' miRNA: 3'- -CGAuGAAG-GCUUGUAAUUGagGUa---GU- -5' |
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| 28218 | 3' | -44.3 | NC_005891.1 | + | 30014 | 0.68 | 0.985542 |
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Target: 5'- aGCUuacguCUUCUGAACcacggccuuGCUCCAUCu -3' miRNA: 3'- -CGAu----GAAGGCUUGuaau-----UGAGGUAGu -5' |
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| 28218 | 3' | -44.3 | NC_005891.1 | + | 13108 | 0.84 | 0.35085 |
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Target: 5'- uUUGCUUCCGAACAUUGACUUUuUCAu -3' miRNA: 3'- cGAUGAAGGCUUGUAAUUGAGGuAGU- -5' |
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| 28218 | 3' | -44.3 | NC_005891.1 | + | 13007 | 1.14 | 0.005222 |
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Target: 5'- aGCUACUUCCGAACAUUAACUCCAUCAa -3' miRNA: 3'- -CGAUGAAGGCUUGUAAUUGAGGUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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