Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2822 | 3' | -50.2 | NC_001491.2 | + | 8658 | 0.67 | 0.989895 |
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Target: 5'- gUUUGGAGAGauugacgaUUGCUCGCUggCaUGGCg -3' miRNA: 3'- aGAACCUUUCg-------AAUGGGCGA--GaACCG- -5' |
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| 2822 | 3' | -50.2 | NC_001491.2 | + | 74562 | 0.67 | 0.988468 |
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Target: 5'- uUCUUGgGGAGGC-UAUuuGCUCaccGGCc -3' miRNA: 3'- -AGAAC-CUUUCGaAUGggCGAGaa-CCG- -5' |
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| 2822 | 3' | -50.2 | NC_001491.2 | + | 16622 | 0.68 | 0.978867 |
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Target: 5'- -gUUGGAccAAGC-UAgCCGCUCUUcguaGGCc -3' miRNA: 3'- agAACCU--UUCGaAUgGGCGAGAA----CCG- -5' |
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| 2822 | 3' | -50.2 | NC_001491.2 | + | 122245 | 0.68 | 0.978867 |
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Target: 5'- cCUUGGggGGUUggcGCuuGCggcgcuUUUUGGCa -3' miRNA: 3'- aGAACCuuUCGAa--UGggCG------AGAACCG- -5' |
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| 2822 | 3' | -50.2 | NC_001491.2 | + | 20685 | 0.69 | 0.95691 |
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Target: 5'- gCUUGGAGAGCUgaucuacaagGCCCGC-CaaGcGCu -3' miRNA: 3'- aGAACCUUUCGAa---------UGGGCGaGaaC-CG- -5' |
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| 2822 | 3' | -50.2 | NC_001491.2 | + | 98911 | 0.7 | 0.948504 |
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Target: 5'- ---cGGcucuGCcgGcCCCGCUCUUGGCg -3' miRNA: 3'- agaaCCuuu-CGaaU-GGGCGAGAACCG- -5' |
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| 2822 | 3' | -50.2 | NC_001491.2 | + | 34669 | 0.71 | 0.928054 |
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Target: 5'- gUCUaGGGAGucGUUUcuccccgGCCCGCUgUUGGCg -3' miRNA: 3'- -AGAaCCUUU--CGAA-------UGGGCGAgAACCG- -5' |
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| 2822 | 3' | -50.2 | NC_001491.2 | + | 96806 | 0.77 | 0.624276 |
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Target: 5'- ---cGGaAAAGUUUGCgUGCUCUUGGCa -3' miRNA: 3'- agaaCC-UUUCGAAUGgGCGAGAACCG- -5' |
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| 2822 | 3' | -50.2 | NC_001491.2 | + | 119183 | 1.14 | 0.004297 |
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Target: 5'- aUCUUGGAAAGCUUACCCGCUCUUGGCa -3' miRNA: 3'- -AGAACCUUUCGAAUGGGCGAGAACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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