Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. |
strand![]() |
Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
2822 | 5' | -56.7 | NC_001491.2 | + | 146364 | 0.67 | 0.82266 |
Target: 5'- cCGGCcacgGCGGcGaGUGAGuccGGCcUCCCCg -3' miRNA: 3'- aGCCGaa--CGCCuC-CAUUC---UCG-AGGGG- -5' |
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2822 | 5' | -56.7 | NC_001491.2 | + | 136326 | 0.67 | 0.82266 |
Target: 5'- gCGGCUcagccuuugGgGGAGGU-AGAGUUUCCa -3' miRNA: 3'- aGCCGAa--------CgCCUCCAuUCUCGAGGGg -5' |
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2822 | 5' | -56.7 | NC_001491.2 | + | 118126 | 0.68 | 0.805338 |
Target: 5'- cUCGGCcuccccggGCGGAGGguccccGGCaCCCCg -3' miRNA: 3'- -AGCCGaa------CGCCUCCauuc--UCGaGGGG- -5' |
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2822 | 5' | -56.7 | NC_001491.2 | + | 119148 | 1.13 | 0.001364 |
Target: 5'- uUCGGCUUGCGGAGGUAAGAGCUCCCCg -3' miRNA: 3'- -AGCCGAACGCCUCCAUUCUCGAGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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