miRNA: 3'- aGCCGaACGCCU---CCAUu--CUCGaGGGG- -5'
miRNA: 3'- aGCCGAa-CGCC---------UCCAUUCUCgaGG-GG- -5'
miRNA: 3'- aGCCGaACGCCUCCauUCUCG------------AGGGG- -5'
miRNA: 3'- -AGCCGAACGCCUCCAUUCUCGAGGGG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2822 | 5' | -56.7 | NC_001491.2 | + | 114302 | 0.71 | 0.619758 |
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Target: 5'- cCGGCgUGUGGAgcuGGUGguuGAGCaCCCCg -3' miRNA: 3'- aGCCGaACGCCU---CCAUu--CUCGaGGGG- -5' |
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| 2822 | 5' | -56.7 | NC_001491.2 | + | 144880 | 0.72 | 0.539203 |
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Target: 5'- cCGGCUggGCGGcagccgccgGGGUAGGAGgaCCgCCg -3' miRNA: 3'- aGCCGAa-CGCC---------UCCAUUCUCgaGG-GG- -5' |
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| 2822 | 5' | -56.7 | NC_001491.2 | + | 19281 | 0.74 | 0.462503 |
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Target: 5'- cUGGC-UGCGGAGGgcAGAGUuuagauaugaguUCUCCa -3' miRNA: 3'- aGCCGaACGCCUCCauUCUCG------------AGGGG- -5' |
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| 2822 | 5' | -56.7 | NC_001491.2 | + | 119148 | 1.13 | 0.001364 |
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Target: 5'- uUCGGCUUGCGGAGGUAAGAGCUCCCCg -3' miRNA: 3'- -AGCCGAACGCCUCCAUUCUCGAGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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