Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28229 | 3' | -46.5 | NC_005893.1 | + | 7227 | 0.66 | 0.98403 |
Target: 5'- aGCACCAGccUUgGUgaucuucucUUGGUCGGAUacagaaagcugugcGGCCu -3' miRNA: 3'- -CGUGGUU--AGgCA---------AACUAGUCUA--------------UCGG- -5' |
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28229 | 3' | -46.5 | NC_005893.1 | + | 9927 | 0.67 | 0.967828 |
Target: 5'- -gGCUAAUCCGUcuauuuUUGAUCGaaucaagAGCCa -3' miRNA: 3'- cgUGGUUAGGCA------AACUAGUcua----UCGG- -5' |
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28229 | 3' | -46.5 | NC_005893.1 | + | 36628 | 0.67 | 0.967828 |
Target: 5'- cGCGCCGuuUuuGga--GUCAGAUAGCUc -3' miRNA: 3'- -CGUGGUu-AggCaaacUAGUCUAUCGG- -5' |
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28229 | 3' | -46.5 | NC_005893.1 | + | 14446 | 0.68 | 0.95571 |
Target: 5'- -uGCCAAUCUGUUcauUCGGcGUAGCUg -3' miRNA: 3'- cgUGGUUAGGCAAacuAGUC-UAUCGG- -5' |
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28229 | 3' | -46.5 | NC_005893.1 | + | 25942 | 0.69 | 0.929275 |
Target: 5'- aGCACCAAUCaGUUUGu----AUAGCCc -3' miRNA: 3'- -CGUGGUUAGgCAAACuagucUAUCGG- -5' |
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28229 | 3' | -46.5 | NC_005893.1 | + | 6859 | 0.75 | 0.643326 |
Target: 5'- gGCACCAGUCuUGUgUGAUUguGGAgAGCCg -3' miRNA: 3'- -CGUGGUUAG-GCAaACUAG--UCUaUCGG- -5' |
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28229 | 3' | -46.5 | NC_005893.1 | + | 26304 | 1.16 | 0.002058 |
Target: 5'- gGCACCAAUCCGUUUGAUCAGAUAGCCu -3' miRNA: 3'- -CGUGGUUAGGCAAACUAGUCUAUCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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