miRNA display CGI


Results 1 - 7 of 7 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
28229 3' -46.5 NC_005893.1 + 7227 0.66 0.98403
Target:  5'- aGCACCAGccUUgGUgaucuucucUUGGUCGGAUacagaaagcugugcGGCCu -3'
miRNA:   3'- -CGUGGUU--AGgCA---------AACUAGUCUA--------------UCGG- -5'
28229 3' -46.5 NC_005893.1 + 9927 0.67 0.967828
Target:  5'- -gGCUAAUCCGUcuauuuUUGAUCGaaucaagAGCCa -3'
miRNA:   3'- cgUGGUUAGGCA------AACUAGUcua----UCGG- -5'
28229 3' -46.5 NC_005893.1 + 36628 0.67 0.967828
Target:  5'- cGCGCCGuuUuuGga--GUCAGAUAGCUc -3'
miRNA:   3'- -CGUGGUu-AggCaaacUAGUCUAUCGG- -5'
28229 3' -46.5 NC_005893.1 + 14446 0.68 0.95571
Target:  5'- -uGCCAAUCUGUUcauUCGGcGUAGCUg -3'
miRNA:   3'- cgUGGUUAGGCAAacuAGUC-UAUCGG- -5'
28229 3' -46.5 NC_005893.1 + 25942 0.69 0.929275
Target:  5'- aGCACCAAUCaGUUUGu----AUAGCCc -3'
miRNA:   3'- -CGUGGUUAGgCAAACuagucUAUCGG- -5'
28229 3' -46.5 NC_005893.1 + 6859 0.75 0.643326
Target:  5'- gGCACCAGUCuUGUgUGAUUguGGAgAGCCg -3'
miRNA:   3'- -CGUGGUUAG-GCAaACUAG--UCUaUCGG- -5'
28229 3' -46.5 NC_005893.1 + 26304 1.16 0.002058
Target:  5'- gGCACCAAUCCGUUUGAUCAGAUAGCCu -3'
miRNA:   3'- -CGUGGUUAGGCAAACUAGUCUAUCGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.