Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28232 | 5' | -43.7 | NC_005893.1 | + | 10389 | 0.66 | 0.996513 |
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Target: 5'- --cUUGCAccgUCACC-AGAAAGCCu-- -3' miRNA: 3'- acuAAUGUa--AGUGGuUCUUUCGGuag -5' |
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| 28232 | 5' | -43.7 | NC_005893.1 | + | 15478 | 0.69 | 0.979752 |
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Target: 5'- uUGGUUAC-UUCugCGcaAGAAAGCCu-- -3' miRNA: 3'- -ACUAAUGuAAGugGU--UCUUUCGGuag -5' |
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| 28232 | 5' | -43.7 | NC_005893.1 | + | 12716 | 0.7 | 0.95854 |
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Target: 5'- cUGGU---AUUCACUAcauGAAGGCCAUCu -3' miRNA: 3'- -ACUAaugUAAGUGGUu--CUUUCGGUAG- -5' |
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| 28232 | 5' | -43.7 | NC_005893.1 | + | 11040 | 0.72 | 0.924925 |
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Target: 5'- aUGAUUGCAccaGCCAgcAGAAuguacggauuggcAGCCAUCa -3' miRNA: 3'- -ACUAAUGUaagUGGU--UCUU-------------UCGGUAG- -5' |
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| 28232 | 5' | -43.7 | NC_005893.1 | + | 25974 | 0.72 | 0.91886 |
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Target: 5'- aUGGUgccUACAUgaCGCCugaaacgauuGAGAAGGCCAUCu -3' miRNA: 3'- -ACUA---AUGUAa-GUGG----------UUCUUUCGGUAG- -5' |
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| 28232 | 5' | -43.7 | NC_005893.1 | + | 15446 | 1.13 | 0.005474 |
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Target: 5'- uUGAUUACAUUCACCAAGAAAGCCAUCa -3' miRNA: 3'- -ACUAAUGUAAGUGGUUCUUUCGGUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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