miRNA: 3'- -CGGAGCC--------ACCGGuacUCGGCGgCGau -5'
miRNA: 3'- -CGGaGCCACCGGUAcuCGGCGGCGau -5'
miRNA: 3'- -CGGAGCCACCGGUACUCGGCGGCGAU- -5'
Results 21 - 23 of 23 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 2824 | 3' | -61.7 | NC_001491.2 | + | 146416 | 0.67 | 0.57853 |
|
Target: 5'- gGCCUCGGgcuguugcUGGCU---GGCCGCgGCa- -3' miRNA: 3'- -CGGAGCC--------ACCGGuacUCGGCGgCGau -5' |
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| 2824 | 3' | -61.7 | NC_001491.2 | + | 129785 | 0.67 | 0.549115 |
|
Target: 5'- aGCCgCGGUGGUCGUu-GCUGCUGUg- -3' miRNA: 3'- -CGGaGCCACCGGUAcuCGGCGGCGau -5' |
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| 2824 | 3' | -61.7 | NC_001491.2 | + | 116969 | 1.08 | 0.000909 |
|
Target: 5'- cGCCUCGGUGGCCAUGAGCCGCCGCUAc -3' miRNA: 3'- -CGGAGCCACCGGUACUCGGCGGCGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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