Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2824 | 5' | -51 | NC_001491.2 | + | 39171 | 0.66 | 0.991402 |
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Target: 5'- cCUCAGAuagcAGcGCUgccCAcgCCcCCAGAGCa -3' miRNA: 3'- -GAGUCU----UCuCGAa--GUa-GGaGGUCUCG- -5' |
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| 2824 | 5' | -51 | NC_001491.2 | + | 140119 | 0.66 | 0.98722 |
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Target: 5'- -gCGGAGGcAGCggacgCgCUCCGGAGCg -3' miRNA: 3'- gaGUCUUC-UCGaaguaG-GAGGUCUCG- -5' |
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| 2824 | 5' | -51 | NC_001491.2 | + | 78673 | 0.67 | 0.979398 |
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Target: 5'- aUCGGc--GGCcaUAUCCUCCAGAGUu -3' miRNA: 3'- gAGUCuucUCGaaGUAGGAGGUCUCG- -5' |
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| 2824 | 5' | -51 | NC_001491.2 | + | 89461 | 0.68 | 0.974359 |
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Target: 5'- aUCAacAGAGCUUCGUCUUCguGAccuaGCc -3' miRNA: 3'- gAGUcuUCUCGAAGUAGGAGguCU----CG- -5' |
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| 2824 | 5' | -51 | NC_001491.2 | + | 147933 | 0.68 | 0.965206 |
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Target: 5'- -cCAGAGGGGCUcgCG-CCgagagccgCCGGGGCg -3' miRNA: 3'- gaGUCUUCUCGAa-GUaGGa-------GGUCUCG- -5' |
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| 2824 | 5' | -51 | NC_001491.2 | + | 148403 | 0.69 | 0.94525 |
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Target: 5'- gUUGGAGGAGCggCAcagCCUCaGGGGCg -3' miRNA: 3'- gAGUCUUCUCGaaGUa--GGAGgUCUCG- -5' |
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| 2824 | 5' | -51 | NC_001491.2 | + | 124534 | 0.7 | 0.935512 |
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Target: 5'- --aGGAAGuaaauaccAGCUUCAUgcggcuggaUCUCCAGAGCu -3' miRNA: 3'- gagUCUUC--------UCGAAGUA---------GGAGGUCUCG- -5' |
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| 2824 | 5' | -51 | NC_001491.2 | + | 38001 | 0.79 | 0.504114 |
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Target: 5'- gUCAuacGGGGCUUCAUCCUCCcccgAGAGCc -3' miRNA: 3'- gAGUcu-UCUCGAAGUAGGAGG----UCUCG- -5' |
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| 2824 | 5' | -51 | NC_001491.2 | + | 116935 | 1.12 | 0.00491 |
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Target: 5'- aCUCAGAAGAGCUUCAUCCUCCAGAGCc -3' miRNA: 3'- -GAGUCUUCUCGAAGUAGGAGGUCUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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