miRNA: 3'- -UUCuCUAAUUaAGGAUUUCGaAGUAA- -5'
miRNA: 3'- -UUCUCuAAUUaAGGAUUUCGaAGUAA- -5'
miRNA: 3'- -UUCUCuAAUUaaGGAUUUCGAAGUaa -5'
miRNA: 3'- -UUCUCUAAUUAAGGAUUUCGAAGUAA- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 28253 | 3' | -40.8 | NC_005902.1 | + | 151246 | 0.68 | 1 |
|
Target: 5'- aAAG-GGUUAAcUCCUAAGGUaUCAUUa -3' miRNA: 3'- -UUCuCUAAUUaAGGAUUUCGaAGUAA- -5' |
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| 28253 | 3' | -40.8 | NC_005902.1 | + | 165833 | 0.72 | 0.999993 |
|
Target: 5'- uAGGAG-UUAAcUCCUAAGGCaUCAUUg -3' miRNA: 3'- -UUCUCuAAUUaAGGAUUUCGaAGUAA- -5' |
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| 28253 | 3' | -40.8 | NC_005902.1 | + | 170637 | 0.7 | 0.999999 |
|
Target: 5'- aAAGAG-UUAAacCCUAAGGCUUCAc- -3' miRNA: 3'- -UUCUCuAAUUaaGGAUUUCGAAGUaa -5' |
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| 28253 | 3' | -40.8 | NC_005902.1 | + | 177716 | 1.05 | 0.150243 |
|
Target: 5'- uAAGAGAUUAAUUCCUAAAGCUUCAUUg -3' miRNA: 3'- -UUCUCUAAUUAAGGAUUUCGAAGUAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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