Results 121 - 129 of 129 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28257 | 3' | -47.8 | NC_005902.1 | + | 161772 | 0.66 | 0.99952 |
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Target: 5'- aAUCCUUUAACaCCuaagGUGUCAUUGAUg -3' miRNA: 3'- -UGGGAAAUUGaGGua--CGCAGUAGUUA- -5' |
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| 28257 | 3' | -47.8 | NC_005902.1 | + | 4541 | 0.66 | 0.999619 |
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Target: 5'- uACCCUUUAACUUCuaaaGCaUUGUCGAUa -3' miRNA: 3'- -UGGGAAAUUGAGGua--CGcAGUAGUUA- -5' |
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| 28257 | 3' | -47.8 | NC_005902.1 | + | 127399 | 0.66 | 0.999699 |
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Target: 5'- aACUCUUUAACUCUuaaGaCGUCAUUGAc -3' miRNA: 3'- -UGGGAAAUUGAGGua-C-GCAGUAGUUa -5' |
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| 28257 | 3' | -47.8 | NC_005902.1 | + | 15221 | 0.66 | 0.999764 |
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Target: 5'- uACCUUUUAACUC---GUGUCAUUGAUg -3' miRNA: 3'- -UGGGAAAUUGAGguaCGCAGUAGUUA- -5' |
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| 28257 | 3' | -47.8 | NC_005902.1 | + | 76344 | 0.66 | 0.999764 |
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Target: 5'- uACUCUUUAACUCCAcaUGa-UCAUUAAc -3' miRNA: 3'- -UGGGAAAUUGAGGU--ACgcAGUAGUUa -5' |
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| 28257 | 3' | -47.8 | NC_005902.1 | + | 31155 | 0.66 | 0.999816 |
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Target: 5'- uACCCUUUAACUCCucuacAUGCugacaacaCAUUAGUg -3' miRNA: 3'- -UGGGAAAUUGAGG-----UACGca------GUAGUUA- -5' |
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| 28257 | 3' | -47.8 | NC_005902.1 | + | 88763 | 0.66 | 0.999816 |
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Target: 5'- aACCCUUUAAUUCUAUauUGUCAaCAAUa -3' miRNA: 3'- -UGGGAAAUUGAGGUAc-GCAGUaGUUA- -5' |
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| 28257 | 3' | -47.8 | NC_005902.1 | + | 154654 | 0.66 | 0.999816 |
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Target: 5'- uACCCUUUAACUUUuaagGCaUUAUCAAc -3' miRNA: 3'- -UGGGAAAUUGAGGua--CGcAGUAGUUa -5' |
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| 28257 | 3' | -47.8 | NC_005902.1 | + | 78150 | 0.66 | 0.999816 |
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Target: 5'- cAUCCUUUAACUUUAcacUGUCAUCAAc -3' miRNA: 3'- -UGGGAAAUUGAGGUac-GCAGUAGUUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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