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miRNA: 3'- -GUCCUCCGUGAGGUGUUGGUCCUAAA- -5'
miRNA: 3'- gUCCUCCGUGAGGUGUUGGUCCUAAA- -5'
miRNA: 3'- guCCUCCGUGAGGUguuggUCCUAAa -5'
miRNA: 3'- -GUCCUCC------GUGAGGugU---UGGUCCUaaa -5'
miRNA: 3'- gucCUCCGUgAGgUGUUGGUCCUAAA- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 28264 | 3' | -54 | NC_005902.1 | + | 61026 | 1.06 | 0.007042 |
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Target: 5'- cCAGGAGGCACUCCACAACCAGGAUUUa -3' miRNA: 3'- -GUCCUCCGUGAGGUGUUGGUCCUAAA- -5' |
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| 28264 | 3' | -54 | NC_005902.1 | + | 61087 | 1.03 | 0.010756 |
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Target: 5'- uGGGAGGCACUCCACAACCAGGAUUUa -3' miRNA: 3'- gUCCUCCGUGAGGUGUUGGUCCUAAA- -5' |
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| 28264 | 3' | -54 | NC_005902.1 | + | 125037 | 0.71 | 0.787421 |
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Target: 5'- -uGGAGGCACUCCAaa---AGGAUUa -3' miRNA: 3'- guCCUCCGUGAGGUguuggUCCUAAa -5' |
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| 28264 | 3' | -54 | NC_005902.1 | + | 72900 | 0.67 | 0.939754 |
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Target: 5'- cCAGGAGGaccauuUAUUCCugGaucACCAGGAg-- -3' miRNA: 3'- -GUCCUCC------GUGAGGugU---UGGUCCUaaa -5' |
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| 28264 | 3' | -54 | NC_005902.1 | + | 60969 | 0.66 | 0.948866 |
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Target: 5'- --aGAGGUAaUCaACAACCAGGAUUUa -3' miRNA: 3'- gucCUCCGUgAGgUGUUGGUCCUAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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