Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2828 | 5' | -55.1 | NC_001491.2 | + | 115587 | 0.66 | 0.927672 |
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Target: 5'- cGCGGccuacUGUccccccGAGCUGGUGGcgGAGCu -3' miRNA: 3'- cCGCU-----ACAc-----CUCGACCACCaaCUCGu -5' |
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| 2828 | 5' | -55.1 | NC_001491.2 | + | 80360 | 0.66 | 0.910516 |
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Target: 5'- uGGCGcgGgucgcUGGGGCUGGUug--GGGCAa -3' miRNA: 3'- -CCGCuaC-----ACCUCGACCAccaaCUCGU- -5' |
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| 2828 | 5' | -55.1 | NC_001491.2 | + | 86920 | 0.67 | 0.869757 |
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Target: 5'- uGGCGGUGUGGgugAGCUugGGcGGggGAgGCGc -3' miRNA: 3'- -CCGCUACACC---UCGA--CCaCCaaCU-CGU- -5' |
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| 2828 | 5' | -55.1 | NC_001491.2 | + | 130583 | 0.68 | 0.854371 |
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Target: 5'- cGGUGGUGUGGucauugaUGGUGGUauguUGGGUu -3' miRNA: 3'- -CCGCUACACCucg----ACCACCA----ACUCGu -5' |
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| 2828 | 5' | -55.1 | NC_001491.2 | + | 121482 | 0.68 | 0.829762 |
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Target: 5'- cGgGAUGagGGGGCUGGUGGac-GGCGu -3' miRNA: 3'- cCgCUACa-CCUCGACCACCaacUCGU- -5' |
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| 2828 | 5' | -55.1 | NC_001491.2 | + | 146179 | 0.68 | 0.821181 |
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Target: 5'- -cCGAUGUGGGGCaGGgGGUUG-GCc -3' miRNA: 3'- ccGCUACACCUCGaCCaCCAACuCGu -5' |
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| 2828 | 5' | -55.1 | NC_001491.2 | + | 120272 | 0.69 | 0.785178 |
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Target: 5'- gGGgGGUGUGGAcGUgGGUGGaaggUGGGUAc -3' miRNA: 3'- -CCgCUACACCU-CGaCCACCa---ACUCGU- -5' |
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| 2828 | 5' | -55.1 | NC_001491.2 | + | 135910 | 0.73 | 0.573096 |
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Target: 5'- uGGUGGgagcUGUGGAGCUGGUuugggaGGUggUGGGUAu -3' miRNA: 3'- -CCGCU----ACACCUCGACCA------CCA--ACUCGU- -5' |
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| 2828 | 5' | -55.1 | NC_001491.2 | + | 114301 | 1.01 | 0.010165 |
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Target: 5'- cGGCG-UGUGGAGCUGGUGGUUGAGCAc -3' miRNA: 3'- -CCGCuACACCUCGACCACCAACUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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