Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28283 | 5' | -38.2 | NC_005902.1 | + | 182513 | 0.66 | 1 |
Target: 5'- uUGAUUC-CUAUAGACUUCu------- -3' miRNA: 3'- -AUUAAGuGAUAUCUGAAGuaaacaaa -5' |
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28283 | 5' | -38.2 | NC_005902.1 | + | 131788 | 0.67 | 1 |
Target: 5'- ----gUugUGUAGACUUCAUaUGUc- -3' miRNA: 3'- auuaaGugAUAUCUGAAGUAaACAaa -5' |
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28283 | 5' | -38.2 | NC_005902.1 | + | 23920 | 0.68 | 1 |
Target: 5'- aAGUUCauaaugACUAUAGACUUUAUucaUUGUa- -3' miRNA: 3'- aUUAAG------UGAUAUCUGAAGUA---AACAaa -5' |
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28283 | 5' | -38.2 | NC_005902.1 | + | 77057 | 0.68 | 1 |
Target: 5'- ---gUCGUUAUAGACUUCAUUcaaUGUa- -3' miRNA: 3'- auuaAGUGAUAUCUGAAGUAA---ACAaa -5' |
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28283 | 5' | -38.2 | NC_005902.1 | + | 38368 | 0.69 | 1 |
Target: 5'- ---gUCAC-AUAGACUUCAUUUa--- -3' miRNA: 3'- auuaAGUGaUAUCUGAAGUAAAcaaa -5' |
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28283 | 5' | -38.2 | NC_005902.1 | + | 80975 | 0.69 | 1 |
Target: 5'- ---gUCAC-AUAGACUUCAUUUa--- -3' miRNA: 3'- auuaAGUGaUAUCUGAAGUAAAcaaa -5' |
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28283 | 5' | -38.2 | NC_005902.1 | + | 90380 | 0.74 | 0.999999 |
Target: 5'- ---gUUGCUAUAGACUUCAUUcgaUGUa- -3' miRNA: 3'- auuaAGUGAUAUCUGAAGUAA---ACAaa -5' |
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28283 | 5' | -38.2 | NC_005902.1 | + | 106612 | 0.82 | 0.995619 |
Target: 5'- ---gUCACUAUAGACUUCAUUUa--- -3' miRNA: 3'- auuaAGUGAUAUCUGAAGUAAAcaaa -5' |
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28283 | 5' | -38.2 | NC_005902.1 | + | 79463 | 1.05 | 0.28891 |
Target: 5'- uUAAUUCACUAUAGACUUCAUUUGUUUu -3' miRNA: 3'- -AUUAAGUGAUAUCUGAAGUAAACAAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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