Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
28287 | 3' | -43.5 | NC_005902.1 | + | 15458 | 0.68 | 1 |
Target: 5'- -aUAUAggUCAGGUGUuACAAGGUuaAUGCUu -3' miRNA: 3'- ccAUAU--GGUCCAUA-UGUUCCA--UAUGG- -5' |
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28287 | 3' | -43.5 | NC_005902.1 | + | 52205 | 0.68 | 1 |
Target: 5'- aGGUAUAUCacuuAGGUGUuaaAGGGUgaauaucuuuuGUACCu -3' miRNA: 3'- -CCAUAUGG----UCCAUAug-UUCCA-----------UAUGG- -5' |
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28287 | 3' | -43.5 | NC_005902.1 | + | 113109 | 0.67 | 1 |
Target: 5'- aGGUAaGCCAGGUgAUAU-AGGUccuCCu -3' miRNA: 3'- -CCAUaUGGUCCA-UAUGuUCCAuauGG- -5' |
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28287 | 3' | -43.5 | NC_005902.1 | + | 169530 | 0.67 | 1 |
Target: 5'- -aUAUAucUCAGGUGUuACAGGGUuaAUGCUu -3' miRNA: 3'- ccAUAU--GGUCCAUA-UGUUCCA--UAUGG- -5' |
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28287 | 3' | -43.5 | NC_005902.1 | + | 80776 | 0.67 | 1 |
Target: 5'- gGGUAUAUagcuUAGGUGUuaaAGGGUaaauauccuucauGUACCa -3' miRNA: 3'- -CCAUAUG----GUCCAUAug-UUCCA-------------UAUGG- -5' |
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28287 | 3' | -43.5 | NC_005902.1 | + | 73074 | 0.68 | 0.999999 |
Target: 5'- uGUAUACCuGGUAUACcuGGcuuuCCu -3' miRNA: 3'- cCAUAUGGuCCAUAUGuuCCauauGG- -5' |
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28287 | 3' | -43.5 | NC_005902.1 | + | 116817 | 0.69 | 0.999993 |
Target: 5'- aGUAUACuUAGGUGUuaAAGGUuaaAUACCc -3' miRNA: 3'- cCAUAUG-GUCCAUAugUUCCA---UAUGG- -5' |
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28287 | 3' | -43.5 | NC_005902.1 | + | 72884 | 0.71 | 0.999916 |
Target: 5'- aGGaAUACCAGGaAUACcAGGaggACCa -3' miRNA: 3'- -CCaUAUGGUCCaUAUGuUCCauaUGG- -5' |
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28287 | 3' | -43.5 | NC_005902.1 | + | 115607 | 0.73 | 0.999237 |
Target: 5'- aGUAUAaguuCCGGGUGaACAaggugaacacauAGGUGUACCu -3' miRNA: 3'- cCAUAU----GGUCCAUaUGU------------UCCAUAUGG- -5' |
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28287 | 3' | -43.5 | NC_005902.1 | + | 73046 | 0.88 | 0.678295 |
Target: 5'- uGGUAUACCucGUAUACcuGGUAUACCu -3' miRNA: 3'- -CCAUAUGGucCAUAUGuuCCAUAUGG- -5' |
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28287 | 3' | -43.5 | NC_005902.1 | + | 113001 | 0.9 | 0.558459 |
Target: 5'- aGGUAUACgAGGUAUACcAGGUAcACCa -3' miRNA: 3'- -CCAUAUGgUCCAUAUGuUCCAUaUGG- -5' |
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28287 | 3' | -43.5 | NC_005902.1 | + | 112974 | 1.19 | 0.019042 |
Target: 5'- aGGUAUACCAGGUAUACAAGGUAUACCa -3' miRNA: 3'- -CCAUAUGGUCCAUAUGUUCCAUAUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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