miRNA: 3'- -AUUGGaGGACUuuGGAU--UACAGaucu -5'
miRNA: 3'- -AUUGGAGGACUUUGGaUUaCAGAu-CU- -5'
miRNA: 3'- aUUGGAGGACUUUGGAUUAcAGAUcu -5'
miRNA: 3'- -AUUGGAGGACUUUGGaUUACAgAUCU- -5'
miRNA: 3'- -AUUGGAGGACUUUGGAUUACAGAUCU- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 28288 | 3' | -46 | NC_005902.1 | + | 92528 | 0.66 | 0.999992 |
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Target: 5'- uUAACCcCCUGAcuCCUAaaGUGUCa--- -3' miRNA: 3'- -AUUGGaGGACUuuGGAU--UACAGaucu -5' |
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| 28288 | 3' | -46 | NC_005902.1 | + | 76926 | 0.71 | 0.995359 |
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Target: 5'- uUAACCUCCUGAAACCcAA-GUUUuuGAa -3' miRNA: 3'- -AUUGGAGGACUUUGGaUUaCAGAu-CU- -5' |
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| 28288 | 3' | -46 | NC_005902.1 | + | 58968 | 0.71 | 0.994564 |
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Target: 5'- aAACUUCCUGAGACCUAGU-UUUAa- -3' miRNA: 3'- aUUGGAGGACUUUGGAUUAcAGAUcu -5' |
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| 28288 | 3' | -46 | NC_005902.1 | + | 109064 | 0.8 | 0.803341 |
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Target: 5'- uUAACCUCUUGAAACCcAAUGUaUAGAg -3' miRNA: 3'- -AUUGGAGGACUUUGGaUUACAgAUCU- -5' |
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| 28288 | 3' | -46 | NC_005902.1 | + | 127026 | 1.09 | 0.029247 |
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Target: 5'- uUAACCUCCUGAAACCUAAUGUCUAGAg -3' miRNA: 3'- -AUUGGAGGACUUUGGAUUACAGAUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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