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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 2830 | 5' | -52.4 | NC_001491.2 | + | 150101 | 0.66 | 0.979359 |
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Target: 5'- -gUCCGGUaguGACGUagGCGGu-AGUGACg -3' miRNA: 3'- gaAGGCCA---CUGCAg-UGUCcuUCACUG- -5' |
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| 2830 | 5' | -52.4 | NC_001491.2 | + | 71597 | 0.68 | 0.954519 |
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Target: 5'- gUUCCGGcccguUGGCGaCACAGGGuuGGUGuuGCg -3' miRNA: 3'- gAAGGCC-----ACUGCaGUGUCCU--UCAC--UG- -5' |
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| 2830 | 5' | -52.4 | NC_001491.2 | + | 17881 | 0.7 | 0.881738 |
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Target: 5'- -gUCUGGgagGuCGUCugAuGGGAGUGGCa -3' miRNA: 3'- gaAGGCCa--CuGCAGugU-CCUUCACUG- -5' |
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| 2830 | 5' | -52.4 | NC_001491.2 | + | 5555 | 0.7 | 0.874439 |
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Target: 5'- aUUCUGGacaUGGCGgCGCAGGAccgucugacaGGUGGCg -3' miRNA: 3'- gAAGGCC---ACUGCaGUGUCCU----------UCACUG- -5' |
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| 2830 | 5' | -52.4 | NC_001491.2 | + | 150152 | 0.81 | 0.328561 |
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Target: 5'- aCUUCCuGUGACGUCACcGGAAGgGGCg -3' miRNA: 3'- -GAAGGcCACUGCAGUGuCCUUCaCUG- -5' |
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| 2830 | 5' | -52.4 | NC_001491.2 | + | 113068 | 1.11 | 0.004827 |
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Target: 5'- cCUUCCGGUGACGUCACAGGAAGUGACg -3' miRNA: 3'- -GAAGGCCACUGCAGUGUCCUUCACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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